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Ch 25: Laboratory Techniques in Molecular Biology: Help & Review

About This Chapter

The Basic Molecular Biology Laboratory Techniques chapter of this AP Biology Help and Review course is the simplest way to master biology lab techniques. This chapter uses simple and fun videos that are about five minutes long, plus lesson quizzes and a chapter exam to ensure students learn the essentials of basic molecular biology laboratory techniques.

Who's it for?

Anyone who needs help learning or mastering AP Biology material will benefit from taking this course. There is no faster or easier way to learn AP Biology. Among those who would benefit are:

  • Students who have fallen behind in understanding genetic engineering and molecular biology laboratory techniques
  • Students who struggle with learning disabilities or learning differences, including autism and ADHD
  • Students who prefer multiple ways of learning science (visual or auditory)
  • Students who have missed class time and need to catch up
  • Students who need an efficient way to learn about molecular biology laboratory techniques
  • Students who struggle to understand their teachers
  • Students who attend schools without extra science learning resources

How it works:

  • Find videos in our course that cover what you need to learn or review.
  • Press play and watch the video lesson.
  • Refer to the video transcripts to reinforce your learning.
  • Test your understanding of each lesson with short quizzes.
  • Verify you're ready by completing the molecular biology laboratory techniques chapter exam.

Why it works:

  • Study Efficiently: Skip what you know, review what you don't.
  • Retain What You Learn: Engaging animations and real-life examples make topics easy to grasp.
  • Be Ready on Test Day: Use the molecular biology laboratory techniques chapter exam to be prepared.
  • Get Extra Support: Ask our subject-matter experts any molecular biology laboratory techniques question. They're here to help!
  • Study With Flexibility: Watch videos on any web-ready device.

Students will review:

This chapter helps students review the concepts in a molecular biology laboratory techniques unit of a standard AP Biology course. Topics covered include:

  • DNA plasmid
  • Agarose gel electrophoresis
  • Bacterial transformation
  • Polymerase chain reaction (PCR)
  • Sanger method for sequencing DNA

14 Lessons in Chapter 25: Laboratory Techniques in Molecular Biology: Help & Review
What is Genetic Engineering? - Definition and Examples

1. What is Genetic Engineering? - Definition and Examples

How do we make the insulin used by diabetic patients? In this lesson, you'll learn the basics of how genetic engineering can be used to transform a bacterial host cell into a genetically-modified organism that produces human insulin.

What is a DNA Plasmid? - Importance to Genetic Engineering

2. What is a DNA Plasmid? - Importance to Genetic Engineering

DNA plasmids play an integral part in most genetic engineering experiments. In this lesson, you'll learn about key features of a plasmid, such as a multiple cloning site, an origin of replication, and a selectable marker.

Restriction Enzymes: Function and Definition

3. Restriction Enzymes: Function and Definition

Restriction enzymes played a critical role in the advent of genetic engineering. In this lesson, you will learn what role restriction enzymes play in creating recombinant DNA.

How Ligase is Used to Engineer Recombinant DNA

4. How Ligase is Used to Engineer Recombinant DNA

DNA ligase makes recombinant DNA technology possible. In this lesson, you will learn how new versions of genes can be designed for experiments in novel host organisms using DNA ligase.

What is Agarose Gel Electrophoresis?

5. What is Agarose Gel Electrophoresis?

Agarose gel electrophoresis plays a key role in genetic engineering experiments. In this lesson, you'll learn what agarose is and how electrophoresis works. You'll also discover the use of agarose in this procedure.

Ethidium Bromide, Loading Buffer & DNA Ladder: Visualizing DNA and Determining its Size

6. Ethidium Bromide, Loading Buffer & DNA Ladder: Visualizing DNA and Determining its Size

In this lesson, you will learn about the role that ethidium bromide, loading buffers, and DNA ladders play in visualizing DNA and determining the size of DNA fragments in agarose gel electrophoresis.

Agarose Gel Electrophoresis: Equipment & Procedure

7. Agarose Gel Electrophoresis: Equipment & Procedure

This lesson will review the concepts and mechanisms of agarose gel electrophoresis. It will also summarize the equipment needed to perform the procedure for DNA analysis.

Agarose Gel Electrophoresis: Results Analysis

8. Agarose Gel Electrophoresis: Results Analysis

Gel electrophoresis is used to analyze DNA restriction digest and ligation experiments. In this lesson, you will learn how to use a DNA ladder to interpret experimental results.

Bacterial Transformation: Definition, Process and Genetic Engineering of E. coli

9. Bacterial Transformation: Definition, Process and Genetic Engineering of E. coli

How can a plasmid be inserted into a bacterial cell? How can transformed bacteria carrying a recombinant plasmid be distinguished from untransformed counterparts? These questions and more will be answered in this lesson.

Bacterial Transformation: Antibiotic Selection and Positive & Negative Controls

10. Bacterial Transformation: Antibiotic Selection and Positive & Negative Controls

The use of antibiotic selection and positive and negative controls are important elements of interpreting data from a bacterial transformation. In this lesson, you will learn how antibiotic selection results in colony formation and how controls help pinpoint the cause of experimental problems.

PCR: Reagents Used in Polymerase Chain Reaction

11. PCR: Reagents Used in Polymerase Chain Reaction

Polymerase chain reaction (PCR) is a biotechnology technique that is used to amplify pieces of DNA. In this lesson, you will learn about five ingredients necessary to perform PCR: template DNA, nucleotides, primers, buffer and Taq polymerase.

PCR: Steps Involved in Polymerase Chain Reaction

12. PCR: Steps Involved in Polymerase Chain Reaction

In this lesson, you will learn about the steps required to amplify DNA during PCR. The lesson will explain the role template DNA, primers, nucleotides, Taq polymerase and PCR buffer play in the denaturation, annealing and extension steps of PCR.

The Sanger Method of DNA Sequencing

13. The Sanger Method of DNA Sequencing

The ability to determine the DNA sequence of an individual is a powerful tool for paternity questions and criminal investigations, among other uses. This lesson will describe one laboratory method that can be used to sequence DNA.

Monomers: Types & Examples

14. Monomers: Types & Examples

Organic molecules, such as proteins, carbohydrates, lipids and nucleic acids, are made of simple subunits called monomers. Learn to identify and describe the different types of monomers that are important to living systems.

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