In preparation for the transfection experiment of DNA manipulation (cloning), discuss how you...

Question:

In preparation for the transfection experiment of DNA manipulation (cloning), discuss how you would assess whether the target gene has any mutations, in comparison to the mutated wild type gene.

Mutations:

Genes are sets of nucleotides that code for a specific protein. These proteins then participate in the biochemical reactions and produce or utilize energy to keep various physiological processes running. Any mutation in the gene is expressed in the protein and can result in the development of disease due to the defective protein.

Answer and Explanation: 1

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During a transfection experiment, the gene is introduced into the subject bacteria with the help of bacteriophages. The gene gets incorporated into...

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Manipulating Heritable Information
Manipulating Heritable Information

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Chapter 14 / Lesson 14
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In this lesson, we'll be learning different strategies that scientists use to change genetic information in cells and organisms. We'll explore how scientists do this and some common applications.


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