Laboratory Techniques in Molecular Biology: Help & Review Chapter Exam

Exam Instructions:

Choose your answers to the questions and click 'Next' to see the next set of questions. You can skip questions if you would like and come back to them later with the yellow "Go To First Skipped Question" button. When you have completed the practice exam, a green submit button will appear. Click it to see your results. Good luck!

Page 1

Question 1 1. Which of the following can be used to visualize DNA bands of an agarose gel?

Question 2 2. Which of the following statements about ethidium bromide are true?

Question 3 3.

Consider the following restriction enzymes and restriction sites. A carot (^) designates the location where the restriction enzyme will cut. Note that only one strand of DNA has been provided.

Acc65I 5'…G^GTACC…3'

EcoRI 5'…G^AATTC…3'

KpnI 5'…GGTAC^C…3'

Which of the following molecules will NOT form hydrogen bonds?

Question 4 4. A scientist believes she has discovered a new nucleic acid dye that is visible to the naked eye. If this is true, which of the following pieces of equipment would no longer be necessary for agarose gel electrophoresis of DNA?

Question 5 5. Which statement is TRUE about the main purpose of an antibiotic selectable marker?'

Page 2

Question 6 6. Which of the following BEST explains the annealing step of PCR?

Question 7 7. Which of the following reagents or equipment are listed in the order in which they would be used to analyze DNA by agarose gel electrophoresis.

Question 8 8. Which of the following is LEAST likely to be used in an experiment in which a human growth hormone gene is inserted into a plasmid?

Question 9 9. Which of the following statements is true?

Question 10 10. Which of the following statements about PCR template DNA is TRUE?

Page 3

Question 11 11. A scientist is testing the effectiveness of a new drug in mice. He knows that the drug works at a concentration of 1 mM, but the dose is lethal. He wishes to assay the effectiveness of the drug at lower concentrations. Which of the following statements is true?

Question 12 12. Ligase could be expected to do which of the following?

Question 13 13. Why is Taq polymerase used in PCR reactions instead of human polymerase?

Question 14 14. Which of the following statement(s) about heat shock are true?

Question 15 15. Which of the following statements is NOT true?

Page 4

Question 16 16. Which of the following statements is TRUE?

Question 17 17. Which of the following statement(s) are true?

Question 18 18. Which of the following features of DNA is primarily responsible for movement of DNA molecules in an electrical field?

Question 19 19. Which of the following statement(s) is FALSE?

Question 20 20. Which of the following would be considered recombinant DNA?

Page 5

Question 21 21. Ligase catalyzes the formation of what type of bond?

Question 22 22. A scientist isolates a human DNA repair gene and inserts it into a plasmid. The plasmid is inserted into a bacterial cell, so more plasmid can be produced. After isolating the plasmid from the bacterial cells, the scientist inserts the plasmid DNA into a human tissue culture cell using a virus. Which of the following statements regarding HOSTS is TRUE?

Question 23 23. Which of the following BEST explains a PCR primer?

Question 24 24. Which of the following represents the correct order of steps in a PCR cycle?

Question 25 25. A scientist is using fluorescently labeled DNA molecules in an agarose gel electrophoresis experiment. A 600-base pair fragment is labeled blue, a 1-kilobase fragment is labeled green, and a 100-base pair fragment is labeled red. Starting from the band closest to the well, which of the following color orders would be expected?

Page 6

Question 26 26. Which of the following statements about calcium chloride's role in transformation is NOT true?

Question 27 27.

Which of the following experiments uses a selectable marker?

I. An experiment in which genetically modified bacteria are blue and all other bacteria are white.

II. An experiment in which the bacteria that has been genetically modified to produce the human insulin protein is identified by checking the DNA sequence.

III. A fruit fly experiment in which a lethal gene kills all progeny except those of the desired genetic makeup.

Question 28 28. A scientist incubates E. coli with iced calcium chloride and a plasmid that contains an ampicillin-resistance gene as the selectable marker. Then, he briefly heat shocks the mixture at forty-two degrees Celsius. After the recommended recovery time, he plates the cells on kanamycin plates. The next day, he is surprised that no cells grew. What statement best explains why the transformation experiment failed?

Question 29 29. Which of the following DNA molecules contains a palindromic sequence?

Question 30 30. Which of the following statements BEST explains the denaturation step?

Laboratory Techniques in Molecular Biology: Help & Review Chapter Exam Instructions

Choose your answers to the questions and click 'Next' to see the next set of questions. You can skip questions if you would like and come back to them later with the yellow "Go To First Skipped Question" button. When you have completed the practice exam, a green submit button will appear. Click it to see your results. Good luck!

AP Biology: Help and Review  /  Science Courses
Support