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AP Biology - Basic Molecular Biology Laboratory Techniques: Tutoring Solution Chapter Exam

Exam Instructions:

Choose your answers to the questions and click 'Next' to see the next set of questions. You can skip questions if you would like and come back to them later with the "Go To First Skipped Question" button. When you have completed the practice exam, a green submit button will appear. Click it to see your results. Good luck!

Page 1

Question 1 1. Based on the following gel, what is the approximate size of the uncut band shown in lane 2?

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Question 2 2.

A piece of DNA was digested with a single restriction enzyme. A portion of the uncut and digested DNA samples were run on the following gel. Lane 1 represents the DNA ladder. Lane 2 represents the uncut DNA sample. And, lane 3 represents the product of the restriction digest. Which of the following statements explains the results?

I. Loading buffer was not added to the DNA samples.

II. The gel was not stained with ethidium bromide.

III. The scientist accidentally used water instead of DNA in lanes 2 and 3.

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Question 3 3. Which of the following BEST explains the annealing step of PCR?

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Question 4 4. Which of the following BEST explains a PCR primer?

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Question 5 5. Consider the DNA molecule below. Which of the following DNA molecules is most likely to bind to the sticky end of this molecule?

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Page 2

Question 6 6. When the R bacteria built a smooth shell from the instructions carried by DNA, we say that it:

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Question 7 7. A scientist is using fluorescently labeled DNA molecules in an agarose gel electrophoresis experiment. A 600-base pair fragment is labeled blue, a 1-kilobase fragment is labeled green, and a 100-base pair fragment is labeled red. Starting from the band closest to the well, which of the following color orders would be expected?

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Question 8 8. Damped oscillations are fluctuations that _____.

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Question 9 9. Why are positive controls important?

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Question 10 10. Starting from the sequencing primer, what is the sequence of the DNA sample?

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Page 3

Question 11 11. The alteration of basic genetic material of an organism is best described as:

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Question 12 12.

Which of the following experiments uses a selectable marker?

I. An experiment in which genetically modified bacteria are blue and all other bacteria are white.

II. An experiment in which the bacteria that has been genetically modified to produce the human insulin protein is identified by checking the DNA sequence.

III. A fruit fly experiment in which a lethal gene kills all progeny except those of the desired genetic makeup.

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Question 13 13. Which of the following are linked together by DNA ligase?

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Question 14 14. A scientist incubates E. coli with iced calcium chloride and a plasmid that contains an ampicillin-resistance gene as the selectable marker. Then, he briefly heat shocks the mixture at forty-two degrees Celsius. After the recommended recovery time, he plates the cells on kanamycin plates. The next day, he is surprised that no cells grew. What statement best explains why the transformation experiment failed?

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Question 15 15. A scientist believes she has discovered a new nucleic acid dye that is visible to the naked eye. If this is true, which of the following pieces of equipment would no longer be necessary for agarose gel electrophoresis of DNA?

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Page 4

Question 16 16. Which of the following statements is NOT true?

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Question 17 17. Consider the following transformation results. Each large circle represents the bacterial growth after an overnight incubation of a successful transformation experiment or successful control. If the scientist was able to conclude that the transformation was successful, which of the following statements concerning the bacteria plated and the type of growth media used are true?

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Question 18 18. What is a DNA Ladder?

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Question 19 19. The following image represents the agarose gel electrophoresis results from a restriction digest experiment. Lane 1 is a DNA ladder and Lane 2 is the DNA sample cut by a single restriction enzyme. What is the MINIMUM size of the uncut DNA fragment, assuming complete digestion has occurred?

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Question 20 20. Which of the following BEST explains the function of Taq polymerase during the extension step of PCR?

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Page 5

Question 21 21. Which of the following statements about PCR template DNA is TRUE?

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Question 22 22. Which of the following DNA molecules contains a palindromic sequence?

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Question 23 23. The transforming principle turned out to be:

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Question 24 24. Which of the following features of DNA is primarily responsible for movement of DNA molecules in an electrical field?

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Question 25 25. When a population goes too far beyond its carrying capacity it will _____.

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Page 6

Question 26 26. Which of the following does the positive control NOT do?

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Question 27 27. Which of the following is included in a Sanger sequencing reaction but not PCR?

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Question 28 28. Functional agents of heredity are called:

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Question 29 29. Which of the following is the main purpose of the origin of replication?

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Question 30 30.

Consider the following restriction enzymes and restriction sites. A carot (^) designates the location where the restriction enzyme will cut. Note that only one strand of DNA has been provided.

Acc65I 5'…G^GTACC…3'

EcoRI 5'…G^AATTC…3'

KpnI 5'…GGTAC^C…3'

Which of the following molecules will NOT form hydrogen bonds?

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AP Biology - Basic Molecular Biology Laboratory Techniques: Tutoring Solution Chapter Exam Instructions

Choose your answers to the questions and click 'Next' to see the next set of questions. You can skip questions if you would like and come back to them later with the "Go To First Skipped Question" button. When you have completed the practice exam, a green submit button will appear. Click it to see your results. Good luck!

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