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AP Biology: Basic Molecular Biology Laboratory Techniques Chapter Exam

Exam Instructions:

Choose your answers to the questions and click 'Next' to see the next set of questions. You can skip questions if you would like and come back to them later with the yellow "Go To First Skipped Question" button. When you have completed the practice exam, a green submit button will appear. Click it to see your results. Good luck!

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Question 1 1. Which of the following statements is TRUE?

Question 2 2. The following image represents a map of a piece of DNA, where each vertical line represents a recognition site for restriction enzyme BamHI. If this DNA was digested completely by BamHI, which of the following agarose gel results would you expect to see?

Question 3 3. Which statement is TRUE about the main purpose of an antibiotic selectable marker?'

Question 4 4. Which of the following features of DNA is primarily responsible for movement of DNA molecules in an electrical field?

Question 5 5. Which of the following BEST explains the function of Taq polymerase during the extension step of PCR?

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Question 6 6. Which statement about loading buffer is true?

Question 7 7.

Which of the following is/are examples of genetically modified organisms?

I. A plant cell in which a regulation gene has been deleted.

II. A bacterial cell in which extra copies of a bacterial DNA repair gene have been added.

III. A human cell that has been infected with a virus.

Question 8 8. Which of the following statements BEST explains a polymerase chain reaction?

Question 9 9. Which of the following are appropriate reagents to add to a human DNA template and PCR buffer to complete the PCR reaction?

Question 10 10. Which of the following statements is FALSE?

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Question 11 11. Which of the following statement(s) is FALSE?

Question 12 12.

All of the samples in the following electrophoresis experiment contain linearized DNA. Which of the following statements could be true?

I. Lane 2 contains the uncut and lane 3 contains the cut samples from a restriction digest experiment.

II. Lane 3 is the unligated DNA and lane 2 is the ligated DNA from the same experiment.

III. Lane 2 and 3 are results of digesting the same DNA sample with different restriction enzymes.

Question 13 13. What is a DNA Ladder?

Question 14 14.

Which of the following accurately represents the order of steps in a transformation experiment?

I. Incubate bacterial cells with chilled CaCl2.

II. Recovery

III. Heat shock

IV. Antibiotic selection

Question 15 15. Which of the following statement(s) about heat shock are true?

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Question 16 16. Which of the following DNA molecules contains a palindromic sequence?

Question 17 17. Ligase catalyzes the formation of what type of bond?

Question 18 18.

Which of the following experiments uses a selectable marker?

I. An experiment in which genetically modified bacteria are blue and all other bacteria are white.

II. An experiment in which the bacteria that has been genetically modified to produce the human insulin protein is identified by checking the DNA sequence.

III. A fruit fly experiment in which a lethal gene kills all progeny except those of the desired genetic makeup.

Question 19 19. Which of the following is the main purpose of the origin of replication?

Question 20 20. Which of the following statements about calcium chloride's role in transformation is NOT true?

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Question 21 21. Which of the following statement(s) are true?

Question 22 22. Which of the following statements are supported by the data provided?

Question 23 23. Starting from the sequencing primer, what is the sequence of the DNA sample?

Question 24 24. Cycling during PCR serves which of the following purposes?

Question 25 25.

A piece of DNA was digested with a single restriction enzyme. A portion of the uncut and digested DNA samples were run on the following gel. Lane 1 represents the DNA ladder. Lane 2 represents the uncut DNA sample. And, lane 3 represents the product of the restriction digest. Which of the following statements explains the results?

I. Loading buffer was not added to the DNA samples.

II. The gel was not stained with ethidium bromide.

III. The scientist accidentally used water instead of DNA in lanes 2 and 3.

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Question 26 26. A scientist isolates a human DNA repair gene and inserts it into a plasmid. The plasmid is inserted into a bacterial cell, so more plasmid can be produced. After isolating the plasmid from the bacterial cells, the scientist inserts the plasmid DNA into a human tissue culture cell using a virus. Which of the following statements regarding VECTORS is TRUE?

Question 27 27. The main purpose of a multiple cloning site is:

Question 28 28. The following three bands were generated by digesting a gene with a single restriction enzyme. A scientist sets up a ligation reaction using all of the products of the restriction digest and analyzes the results of the ligation at varying durations of time. Which of the following gel results could not be produced by this experiment?

Question 29 29.

Consider the following restriction enzymes and restriction sites. A carot (^) designates the location where the restriction enzyme will cut. Note that only one strand of DNA has been provided.

Acc65I 5'…G^GTACC…3'

EcoRI 5'…G^AATTC…3'

KpnI 5'…GGTAC^C…3'

Which of the following molecules will NOT form hydrogen bonds?

Question 30 30. The following image represents the agarose gel electrophoresis results from a restriction digest experiment. Lane 1 is a DNA ladder and Lane 2 is the DNA sample cut by a single restriction enzyme. What is the MINIMUM size of the uncut DNA fragment, assuming complete digestion has occurred?

AP Biology: Basic Molecular Biology Laboratory Techniques Chapter Exam Instructions

Choose your answers to the questions and click 'Next' to see the next set of questions. You can skip questions if you would like and come back to them later with the yellow "Go To First Skipped Question" button. When you have completed the practice exam, a green submit button will appear. Click it to see your results. Good luck!

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