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Basic Molecular Biology Laboratory Techniques: Homework Help Chapter Exam

Exam Instructions:

Choose your answers to the questions and click 'Next' to see the next set of questions. You can skip questions if you would like and come back to them later with the yellow "Go To First Skipped Question" button. When you have completed the practice exam, a green submit button will appear. Click it to see your results. Good luck!

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Question 1 1. Cycling during PCR serves which of the following purposes?

Question 2 2. Starting from the sequencing primer, what is the sequence of the DNA sample?

Question 3 3. The following three bands were generated by digesting a gene with a single restriction enzyme. A scientist sets up a ligation reaction using all of the products of the restriction digest and analyzes the results of the ligation at varying durations of time. Which of the following gel results could not be produced by this experiment?

Question 4 4.

Which of the following accurately represents the order of steps in a transformation experiment?

I. Incubate bacterial cells with chilled CaCl2.

II. Recovery

III. Heat shock

IV. Antibiotic selection

Question 5 5.

Consider the following restriction enzymes and restriction sites. A carot (^) designates the location where the restriction enzyme will cut. Note that only one strand of DNA has been provided.

Acc65I 5'…G^GTACC…3'

EcoRI 5'…G^AATTC…3'

KpnI 5'…GGTAC^C…3'

Which of the following molecules will NOT form hydrogen bonds?

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Question 6 6. Which of the following statement(s) is FALSE?

Question 7 7. Which of the following BEST explains a PCR primer?

Question 8 8. The following image represents a map of a piece of DNA, where each vertical line represents a recognition site for restriction enzyme BamHI. If this DNA was digested completely by BamHI, which of the following agarose gel results would you expect to see?

Question 9 9. Consider the following transformation results. Each large circle represents the bacterial growth after an overnight incubation of a successful transformation experiment or successful control. If the scientist was able to conclude that the transformation was successful, which of the following statements concerning the bacteria plated and the type of growth media used are true?

Question 10 10. Which of the following BEST explains the function of Taq polymerase during the extension step of PCR?

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Question 11 11. Which of the following statements is FALSE?

Question 12 12. Which of the following statements about ethidium bromide are true?

Question 13 13. Which of the following statement(s) about heat shock are true?

Question 14 14. Which of the following are examples of genetically modified organisms?

Question 15 15. Which of the following statements about PCR template DNA is TRUE?

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Question 16 16. A scientist is testing the effectiveness of a new drug in mice. He knows that the drug works at a concentration of 1 mM, but the dose is lethal. He wishes to assay the effectiveness of the drug at lower concentrations. Which of the following statements is true?

Question 17 17. Which of the following features of DNA is primarily responsible for movement of DNA molecules in an electrical field?

Question 18 18. Why is Taq polymerase used in PCR reactions instead of human polymerase?

Question 19 19. Which of the following statements is true?

Question 20 20. Which of the following statements is TRUE?

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Question 21 21. Which of the following statements about calcium chloride's role in transformation is NOT true?

Question 22 22. A scientist is using fluorescently labeled DNA molecules in an agarose gel electrophoresis experiment. A 600-base pair fragment is labeled blue, a 1-kilobase fragment is labeled green, and a 100-base pair fragment is labeled red. Starting from the band closest to the well, which of the following color orders would be expected?

Question 23 23.

All of the samples in the following electrophoresis experiment contain linearized DNA. Which of the following statements could be true?

I. Lane 2 contains the uncut and lane 3 contains the cut samples from a restriction digest experiment.

II. Lane 3 is the unligated DNA and lane 2 is the ligated DNA from the same experiment.

III. Lane 2 and 3 are results of digesting the same DNA sample with different restriction enzymes.

Question 24 24. Consider the following electrophoresis gel. A scientist wishes to isolate a gene that is 1.6 kb in size. Which of the following DNA bands could contain the intact gene?

Question 25 25. What is the difference between a deoxyribonucleotide and a dideoxyribonucleotide?

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Question 26 26.

Which of the following experiments uses a selectable marker?

I. An experiment in which genetically modified bacteria are blue and all other bacteria are white.

II. An experiment in which the bacteria that has been genetically modified to produce the human insulin protein is identified by checking the DNA sequence.

III. A fruit fly experiment in which a lethal gene kills all progeny except those of the desired genetic makeup.

Question 27 27. When setting up a Sanger sequencing reaction, each reaction should include template DNA, nucleotides, dideoxynucleotides, buffer, DNA polymerase, and _____.

Question 28 28. Which of the following are linked together by DNA ligase?

Question 29 29. A scientist believes she has discovered a new nucleic acid dye that is visible to the naked eye. If this is true, which of the following pieces of equipment would no longer be necessary for agarose gel electrophoresis of DNA?

Question 30 30. Which of the following is LEAST likely to be used in an experiment in which a human growth hormone gene is inserted into a plasmid?

Basic Molecular Biology Laboratory Techniques: Homework Help Chapter Exam Instructions

Choose your answers to the questions and click 'Next' to see the next set of questions. You can skip questions if you would like and come back to them later with the yellow "Go To First Skipped Question" button. When you have completed the practice exam, a green submit button will appear. Click it to see your results. Good luck!

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