Basic Molecular Biology Laboratory Techniques Chapter Exam

Exam Instructions:

Choose your answers to the questions and click 'Next' to see the next set of questions. You can skip questions if you would like and come back to them later with the yellow "Go To First Skipped Question" button. When you have completed the practice exam, a green submit button will appear. Click it to see your results. Good luck!

Page 1

Question 1 1. Which of the following is not an application of recombinant DNA technology?

Question 2 2. Which of the following statement(s) is FALSE?

Question 3 3. A scientist believes she has discovered a new nucleic acid dye that is visible to the naked eye. If this is true, which of the following pieces of equipment would no longer be necessary for agarose gel electrophoresis of DNA?

Question 4 4. Based on the following gel, what is the size of the band in lane 2?

Question 5 5. What is a cDNA clone?

Page 2

Question 6 6. Which of the following is considered a vector?

Question 7 7. A scientist incubates E. coli with iced calcium chloride and a plasmid that contains an ampicillin-resistance gene as the selectable marker. Then, he briefly heat shocks the mixture at forty-two degrees Celsius. After the recommended recovery time, he plates the cells on kanamycin plates. The next day, he is surprised that no cells grew. What statement best explains why the transformation experiment failed?

Question 8 8. Which of the following statements about PCR template DNA is TRUE?

Question 9 9. At the end of a bacterial transformation, the scientist observes the following results: a lawn on both the antibiotic and non-antibiotic plates regardless of the transformation state of the bacteria plated. Choose the statement that best describes the experimental results.

Question 10 10. Which of the following are appropriate reagents to add to a human DNA template and PCR buffer to complete the PCR reaction?

Page 3

Question 11 11.

Which of the following accurately represents the order of steps in a transformation experiment?

I. Incubate bacterial cells with chilled CaCl2.

II. Recovery

III. Heat shock

IV. Antibiotic selection

Question 12 12. Which of the following reagents or equipment are listed in the order in which they would be used to analyze DNA by agarose gel electrophoresis.

Question 13 13. Starting from the sequencing primer, what is the sequence of the DNA sample?

Question 14 14. The following image represents the agarose gel electrophoresis results from a restriction digest experiment. Lane 1 is a DNA ladder and Lane 2 is the DNA sample cut by a single restriction enzyme. What is the MINIMUM size of the uncut DNA fragment, assuming complete digestion has occurred?

Question 15 15. Cloning a gene may involve which of these processes?

Page 4

Question 16 16. Which of the following statements is NOT true?

Question 17 17. Cycling during PCR serves which of the following purposes?

Question 18 18. Which of the following statements about ethidium bromide are true?

Question 19 19. The following three bands were generated by digesting a gene with a single restriction enzyme. A scientist sets up a ligation reaction using all of the products of the restriction digest and analyzes the results of the ligation at varying durations of time. Which of the following gel results could not be produced by this experiment?

Question 20 20. For a prokaryotic vector to be propagated in a host bacterial cell, the vector needs

Page 5

Question 21 21. Which of the following is the main purpose of the origin of replication?

Question 22 22. Which of these can inhibit gene expression?

Question 23 23. Expression vectors are useful for what?

Question 24 24. RNAi

Question 25 25. Which of the following would be considered recombinant DNA?

Page 6

Question 26 26.

Which of the following experiments uses a selectable marker?

I. An experiment in which genetically modified bacteria are blue and all other bacteria are white.

II. An experiment in which the bacteria that has been genetically modified to produce the human insulin protein is identified by checking the DNA sequence.

III. A fruit fly experiment in which a lethal gene kills all progeny except those of the desired genetic makeup.

Question 27 27. Ho do expression vectors differ from other vectors?

Question 28 28. Which of the following BEST explains the annealing step of PCR?

Question 29 29. What is a DNA Ladder?

Question 30 30. Which of the following are types of reporter genes?

Basic Molecular Biology Laboratory Techniques Chapter Exam Instructions

Choose your answers to the questions and click 'Next' to see the next set of questions. You can skip questions if you would like and come back to them later with the yellow "Go To First Skipped Question" button. When you have completed the practice exam, a green submit button will appear. Click it to see your results. Good luck!

MCAT Test: Practice and Study Guide  /  Standardized Tests Courses
Support