Holt McDougal Modern Biology Chapter 13: Gene Technology Chapter Exam - Exam Results

Exam Instructions:

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Question 1 1. Which of the following are linked together by DNA ligase?

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Question 2 2. Which of the following statements is TRUE?

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Question 3 3.

Consider the following restriction enzymes and restriction sites. A carot (^) designates the location where the restriction enzyme will cut. Note that only one strand of DNA has been provided.

Acc65I 5'…G^GTACC…3'

EcoRI 5'…G^AATTC…3'

KpnI 5'…GGTAC^C…3'

Which of the following molecules will NOT form hydrogen bonds?

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Question 4 4. Which of the following is the main purpose of the origin of replication?

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Question 5 5. The main purpose of a multiple cloning site is:

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Question 6 6.

Which of the following experiments uses a selectable marker?

I. An experiment in which genetically modified bacteria are blue and all other bacteria are white.

II. An experiment in which the bacteria that has been genetically modified to produce the human insulin protein is identified by checking the DNA sequence.

III. A fruit fly experiment in which a lethal gene kills all progeny except those of the desired genetic makeup.

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Question 7 7.

Which of the following is/are examples of genetically modified organisms?

I. A plant cell in which a regulation gene has been deleted.

II. A bacterial cell in which extra copies of a bacterial DNA repair gene have been added.

III. A human cell that has been infected with a virus.

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Question 8 8. A scientist isolates a human DNA repair gene and inserts it into a plasmid. The plasmid is inserted into a bacterial cell, so more plasmid can be produced. After isolating the plasmid from the bacterial cells, the scientist inserts the plasmid DNA into a human tissue culture cell using a virus. Which of the following statements regarding VECTORS is TRUE?

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Question 9 9. Which of the following statements is NOT true?

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Question 10 10. If the recognition sequence of the restriction enzyme HindIII is AAGCTT, then how many covalent bonds will be broken by the enzyme in the following DNA molecule?

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Question 11 11. Which of the following statements is TRUE?

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Question 12 12. Which of the following statements is FALSE?

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Question 13 13. What might be used to clone DNA instead of bacteria?

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Question 14 14. Which of the following is a product of DNA cloning?

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Question 15 15. The enzyme used to 'paste' together the DNA strands is called

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Question 16 16. What was the Human Genome Project?

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Question 17 17. What is proteomics?

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Question 18 18. What did J. Craig Venter do?

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Question 19 19.

A scientist wishes to verify that a restriction digestion has successfully cut a linear DNA fragment. She decides to compare the cut and uncut DNA samples using agarose gel electrophoresis. Which of the following agarose gel results is a result that would indicate the linear piece of DNA was digested?

I. One band in the uncut DNA lane and two smaller bands in the digested DNA lane

II. One band in the uncut DNA lane and a single smaller band in the digested DNA lane

III. One band in the uncut DNA lane and three smaller bands in the digested DNA lane

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Question 20 20. Which of the following statements is true?

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Question 21 21. Which of the following features of DNA is primarily responsible for movement of DNA molecules in an electrical field?

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Question 22 22. Which of the following BEST explains the annealing step of PCR?

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Question 23 23. PCR Cycling serves which of the following purposes?

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Question 24 24. Which of the following BEST explains the function of Taq polymerase during the extension step of PCR?

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Question 25 25. Which of the following is NOT an issue associated with genetic engineering?

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Question 26 26. Which set of crops has been the primary focus of genetic engineering?

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Question 27 27. In agriculture, how has genetic engineering helped to manage unwanted insects?

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Question 28 28. Why is gene therapy controversial?

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Question 29 29. Which of the following is NOT a current use of gene therapy?

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Question 30 30. Which of the following is NOT a way that genes can be introduced into the body in gene therapy?

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Holt McDougal Modern Biology Chapter 13: Gene Technology Chapter Exam Instructions

Choose your answers to the questions and click 'Next' to see the next set of questions. You can skip questions if you would like and come back to them later with the "Go To First Skipped Question" button. When you have completed the practice exam, a green submit button will appear. Click it to see your results. Good luck!

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