RNA Processing in a Eukaryotic Cell: Splicing of Introns & Exons

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  • 0:05 Gene Regulation in Eukaryotes
  • 1:16 Introns and Exons
  • 2:21 RNA Splicing and Spliceosomes
  • 4:37 The 5' Cap and the…
  • 6:23 The Maturation of mRNA
  • 7:52 Lesson Summary
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Lesson Transcript
Instructor: April Koch

April teaches high school science and holds a master's degree in education.

In this lesson, we'll explore the unique considerations for gene regulation in the eukaryotic cell. We'll walk through RNA splicing of introns and exons and the addition of the 5' cap and poly(A) tail.

Gene Regulation in Eukaryotes

All living things have methods for regulating their genes by controlling when and how their genes are expressed. When scientists first learned about gene regulation, they studied the bacterium E. coli and one of its gene clusters: the lac operon. Scientists used the lac operon to study gene regulation. Their research revealed plenty of information about the regulation of operons, but it didn't tell them much about the regulation of human genes. After all, we humans don't have operons. Humans, remember, are eukaryotes.

Unlike the prokaryotic bacteria, eukaryotes don't have clusters of related genes. Our genes are scattered throughout the genome over multiple chromosomes. Also, our cells contain a nucleus, meaning that we have to perform transcription and translation separately. Transcription has to happen in the nucleus, but translation must occur in the cytoplasm. So, unlike prokaryotes, eukaryotes undergo an RNA processing step right after transcription and before translation. RNA processing is a challenge that's unique to eukaryotes because only eukaryotic cells have a nucleus. In this lesson, we'll walk through the most important aspects of RNA processing.

Introns and Exons

Eukaryotic genes are split up into parts that are expressed as RNA products and parts that are not. The parts that are expressed are called exons. They appear in short sequences scattered throughout the gene. Exons are the parts of a eukaryotic gene that are expressed through transcription and translation. You can think of them like the 'meat' of the gene; they're typically considered the actual coding regions that specify our proteins.

In between all the exons lie the other segments, called introns. Introns are the intervening sequences that are removed from a gene before the RNA product is made. Introns and exons alternate with each other along the length of a gene. Introns are usually considered non-coding regions because they don't seem to code for any enzymes or structural proteins. However, scientists are still learning about the importance of introns. One thing we do know is that introns are removed from an RNA sequence right after transcription. They exist in DNA, they're transcribed into RNA, but then they're taken out before the RNA has a chance to make any products.

Introns are removed from the RNA sequence after transcription

RNA Splicing and Spliceosomes

So, exons hold the important coding regions of our genes, and introns aren't much more than extra fluff. How do we get hold of only the good stuff? How do we separate the wheat from the chaff? The RNA strand is processed so that its introns are removed and the exons are pushed together to make a continuous, shorter strand. This process is called RNA splicing. It produces a 'final draft' of the mRNA before translation gets under way. RNA splicing is the removal of introns and joining of exons in eukaryotic mRNA. It also occurs in tRNA and rRNA.

Splicing is accomplished with the help of spliceosomes, which remove introns from the genes in RNA. Spliceosomes are composed of a mixture of protein and small RNA molecules. They locate the ends of the introns, cut them away from the exons, and join the ends of adjacent exons together. Once the entire gene is devoid of its introns, the process of RNA splicing is complete.

What is the point of RNA splicing? Why do we have introns in the first place if we're only going to remove them later? When scientists first discovered introns, they didn't think there was any reason for having them in our genes. They originally called the introns 'junk DNA.' But, further research revealed that introns play an important role in gene expression. They separate the coding regions of the gene and allow different combinations of exons to be spliced together. In other words, a single gene is capable of coding for more than one protein. This principle is called alternative splicing.

Take a look at the sample eukaryotic gene below. It has four different segments that can be spliced in alternative combinations. One combination counts the green segment as an intron and removes it from the mRNA. The resulting protein is translated only from the red, yellow, and blue gene segments. A different combination counts the yellow segment as a removable intron and the green segment as a translatable exon. Each combination yields a different protein, both of which were translated from the same gene. Scientists are just beginning to understand the possibilities presented by alternative splicing. Therefore, we are still learning about the role of introns in our genes.

Introns play an important role in gene expression and allow for alternative splicing
Alternative Splicing

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