The Tests Used
Saliva is the one fluid we'll discuss that only has one possible testing procedure. This test does not have a name; it simply involves placing the suspected saliva in a mixture of iodine and starch. If only iodine and starch are mixed, they will create a deep purple color. When saliva is introduced to the mixture, the color will begin to fade. So, say a small stain was found on the collar of a victim's blouse. It's suspected to be saliva, but has already dried. The stained piece of fabric can be placed in the mixture of iodine and starch to determine if saliva is actually present.
Semen is a bodily fluid whose presence can be confirmed through two specific tests. A UV light can be held over suspected semen. Since semen will turn fluorescent under UV lighting, the UV light allows us to see semen if it's there. In another test, the suspected semen can be touched with a solution of acid phosphatase. If semen is present, the color will change to purple. Both of these tests can be performed on stains and actual fluid. For example, a medical examiner may gather suspected semen during a sexual assault exam and touch it with a cotton swab dipped in acid phosphatase. The same medical examiner may also see a dried-up spot on the inside of a victim's thigh and simply shine a UV light over the spot to see if it fluoresces.
Finally, there are a few tests that determine the presence of blood. These include the use of Kastle-Meyer, Leucho-Malachite Green, and Luminol solutions. Each of these will cause a different reaction when met with blood. The Kastle-Meyer solution will turn pink, the Leucho-Malachite solution will turn blue-green, and the Luminol will glow when it makes contact with blood. For Luminol to glow, it must be sprayed on a suspected stain and then viewed under darkness. For example, a crime scene may have large areas where there seems to be blood. A detective could spray Luminol on the spots, turn off the lights, and see if the spots glow. If they do, blood may be present.
All these tests do is simply determine the presence of bodily fluids. If bodily fluids are found, they can be used to extract DNA, which may identify a perpetrator. If it does not identify the perpetrator initially, the information can be placed in a database to be matched to other crimes later on if the same perpetrator commits crimes multiple times.
Preservation Is Important
In these tests, preservation is important. One way to preserve evidence like bodily fluids is the smear method. In this method, the bodily fluids are suspended in another fluid. That fluid can be placed on a slide later on and viewed under a microscope. Bodily fluids may also be scraped or sucked from a surface, such as when the sample is already dry. In either method of preserving the fluid, it may be later smeared onto a slide and viewed under a microscope.
Lesson Summary
Once again, serology tests are important in the field of forensic serology, which is the study of bodily fluids used in the investigation of a criminal matter. These fluids include blood, semen, or saliva, and different tests are used to find each one. Kastle-Meyer, Leucho-Malachite Green, and Luminol tests are used to determine the presence of blood. A mixture of iodine and starch may be used to determine the presence of saliva. And, semen can be found using UV lights or an acid phosphatase solution. Once these tests have uncovered the presence of a bodily fluid, investigators may be able to extract DNA from the fluids to determine the identity of the perpetrator.
