What is Agarose Gel Electrophoresis?


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question 1 of 3

A scientist wishes to verify that a restriction digestion has successfully cut a linear DNA fragment. She decides to compare the cut and uncut DNA samples using agarose gel electrophoresis. Which of the following agarose gel results is a result that would indicate the linear piece of DNA was digested?

I. One band in the uncut DNA lane and two smaller bands in the digested DNA lane

II. One band in the uncut DNA lane and a single smaller band in the digested DNA lane

III. One band in the uncut DNA lane and three smaller bands in the digested DNA lane

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1. Which of the following features of DNA is primarily responsible for movement of DNA molecules in an electrical field?

2. Which of the following statements is true?

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About This Quiz & Worksheet

This quiz and worksheet will test your understanding of agarose gel electrophoresis. To pass the quiz you must be knowledgeable of how the electrophoresis process works and other information included in the lesson.

Quiz & Worksheet Goals

Assessments will test you on the following:

  • Facts about DNA fragments
  • The process of gel electrohphoresis
  • The separation of DNA fragments

Skills Practiced

Practiced skills include the following:

  • Information Recall- access the knowledge you've gained regarding the color order of a kilobase
  • Interpreting Information- verify that you can read information regarding the movement of DNA fragments during the electrophoresis process
  • Reading Comprehension- ensure that you draw the most important information from the related lesson on agarose gel electrohphoresis

Additional Learning

To learn more about agarose gel electrophoresis, review the lesson, What is Agarose Gel Electrophoresis? Objectives covered include:

  • Review genetic engineering
  • Understand the mechanisms of electrophoresis
  • Define Agarose
  • Know how gel electrophoresis works