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Agarose Gel Electrophoresis: Results Analysis

Instructions:

Choose an answer and hit 'next'. You will receive your score and answers at the end.

question 1 of 3

The following image represents the agarose gel electrophoresis results from a restriction digest experiment. Lane 1 is a DNA ladder and Lane 2 is the DNA sample cut by a single restriction enzyme. What is the MINIMUM size of the uncut DNA fragment, assuming complete digestion has occurred?

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1. The following image represents a map of a piece of DNA, where each vertical line represents a recognition site for restriction enzyme BamHI. If this DNA was digested completely by BamHI, which of the following agarose gel results would you expect to see?

2. The following image represents the agarose gel results from the restriction digest of a 6 kb piece of DNA that possesses two restriction sites. Which of the following statements is true?

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About This Quiz & Worksheet

These interactive learning assessments help test your knowledge of agarose gel electrophoresis. You'll be asked several questions on how various experiment results can be interpreted.

Quiz & Worksheet Goals

This quiz and worksheet combo will ask you to analyze the results of various gel electrophoresis experiments considering:

  • DNA fragments
  • DNA ladders
  • Restriction digests
  • Restriction enzymes
  • Ligation reactions

Skills Practiced

These assessments will test you on the following skills:

  • Defining key concepts - know the correct terminology used in gel electrophoresis procedures
  • Information recall - access and apply what you already know about gel electrophoresis to answer questions about experiment results
  • Distinguishing differences - see how different gel electrophoresis experiment results yield different analyses

Additional Learning

To learn more about agarose gel electrophoresis, watch the accompanying lesson on Analyzing Results of Agarose Gel Electrophoresis. The lesson covers the following:

  • Define agarose gel electrophoresis
  • Understand the purpose of gel electrophoresis
  • Learn how DNA ladders verify size of DNA fragments
  • Consider different questions used to analyze experiment results
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