Bacterial Transformation: Antibiotic Selection and Positive & Negative Controls


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question 1 of 3

A scientist performs antibiotic selection following a bacterial transformation experiment. After incubating the bacterial at 37 degrees Celsius overnight, she finds half a dozen colonies on the plate. Consider which of the following statements are true.

I. A successful experiment would have produced a lawn rather than a handful of colonies.

II. Six cells were successfully transformed.

III. Since there are six colonies, the scientist must have started the experiment with six cells.

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1. A scientist is testing the effectiveness of a new drug in mice. He knows that the drug works at a concentration of 1 mM, but the dose is lethal. He wishes to assay the effectiveness of the drug at lower concentrations. Which of the following statements is true?

2. At the end of a bacterial transformation, the scientist observes the following results: a lawn on both the antibiotic and non-antibiotic plates regardless of the transformation state of the bacteria plated. Choose the statement that best describes the experimental results.

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About This Quiz & Worksheet

The lesson that accompanies this chapter covers some procedures and controls of bacterial and antibiotic research. These assessments have been paired with this lesson to measure your understanding of experiments involving antibiotic selection and bacterial transformation. The quiz questions will provide you with sample experiments to interpret.

Quiz & Worksheet Goals

This worksheet/quiz set will test your skills on the following:

  • Assessing the success of bacterial cultivation
  • Implementing positive controls
  • Interpreting the results from bacterial cultivations

Skills Practiced

Completing these assessments will also test your other skills, including:

  • Knowledge application - use your knowledge to answer questions about research with bacterial colonies
  • Information recall - access the knowledge you've gained regarding antibiotic selection
  • Making connections - use your understanding of the concepts of positive and negative controls

Additional Learning

Ensure you know what you need to know about this type of experiment by reviewing the lesson that accompanies these assessments, Bacterial Transformation: Antibiotic Selection and Positive & Negative Controls. The objective of this review is to improve your knowledge about the following:

  • Growing bacterial colonies
  • Understanding experiment control
  • Implementing positive and negative controls
  • Recognizing the significance of bacterial transformation