Bacterial Transformation: Definition, Process and Genetic Engineering of E. coli


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question 1 of 3

Which of the following statements about E. coli is NOT true?

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Which of the following accurately represents the order of steps in a transformation experiment?

I. Incubate bacterial cells with chilled CaCl2.

II. Recovery

III. Heat shock

IV. Antibiotic selection

2. Which of the following statements about calcium chloride's role in transformation is NOT true?

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About This Quiz & Worksheet

This worksheet and accompanying quiz will help to test your knowledge of the process of bacterial transformation, including both the steps and chemicals involved. Some of the terms you will be tested on include E. coli, calcium chloride, and heat shock.

Quiz & Worksheet Goals

In these assessments you will be tested on:

  • The properties of E. coli
  • The steps involved in bacterial transformation
  • The role of calcium chloride in transformation
  • Heat shock
  • Evaluating an example of a transformation experiment

Skills Practiced

This quiz/worksheet combo will allow students to test the following skills:

  • Making connections - use understanding of the concept of bacterial transformation
  • Defining key concepts - ensure that you can accurately define main phrases, such as heat shock
  • Knowledge application - use your knowledge to answer questions about genetically modifying bacterial cells

Additional Learning

To learn more, review the accompanying lesson on Bacterial Transformation. This lesson covers the follow objectives:

  • Define transformation and heat shock
  • Understand the properties of E. coli and calcium chloride
  • Understand the process of bacterial transformation