Bradford Protein Assay: Principle, Protocol & Calculations

Instructions:

Choose an answer and hit 'next'. You will receive your score and answers at the end.

question 1 of 3

What amino acids does the Bradford Protein Assay primarily measure?

Create Your Account To Take This Quiz

As a member, you'll also get unlimited access to over 75,000 lessons in math, English, science, history, and more. Plus, get practice tests, quizzes, and personalized coaching to help you succeed.

Try it risk-free
Try it risk-free for 30 days. Cancel anytime.
Already registered? Log in here for access

1. What color change occurs when proteins combine with Coomassie dye under acidic conditions?

Use the provided material to answer questions 2-4.

Using BSA concentrations of 0 mcg/mL, 1 mcg/mL, 3 mcg/mL, 4 mcg/mL, and 5 mcg/mL you get the following absorptions: 0.02, 0.12, 0.32, 0.42, 0.52.

With your sample you dilute it 1:5 and get an absorbance of 0.15.

2. What is the standard curve equation for this BSA standard?

Create your account to access this entire worksheet
A Premium account gives you access to all lesson, practice exams, quizzes & worksheets
Access to all video lessons
Quizzes, practice exams & worksheets
Certificate of Completion
Access to instructors
Create an account to get started Create Account

About This Quiz & Worksheet

Test how well you understand the calculations and protocols associated with the Bradford Protein Assay by taking this short quiz. You'll be asked about the amino acids measured by the Bradford Protein Assay and how to find several different variables of the equation used to calculate it.

Quiz & Worksheet Goals

Our helpful quiz tests your understanding of how to:

  • Identify the amino acids that the Bradford Protein Assay measures
  • Describe the color change that occurs when proteins combine with Coomassie dye under acidic conditions
  • Select the correct standard curve equation for an example BSA standard
  • Decipher the components of a standard curve equation when given example data
  • Calculate the protein concentration of an example

Skills Practiced

  • Information recall - access the knowledge you've gained regarding the color change that happens when proteins combine with Coomassie dye in acidic conditions
  • Reading comprehension - ensure that you draw the most important information from the related Bradford Protein Assay lesson
  • Critical thinking - apply relevant concepts to examine information about the calculations used for the Bradford Protein Assay in a different light

Additional Learning

If you'd like to study this subject further, take a look at the lesson called Bradford Protein Assay: Principle, Protocol & Calculations for more information. In addition to helping you answer the questions on this quiz, you'll also learn how to:

  • Identify the tool used to measure the level of blue in the test
  • Explain when the Bradford Protein Assay might not be the best test to use
  • Detail the substance commonly used as the standard in the assay
Support