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Enzyme-Linked Immunosorbent Assay: Definition & Application

Instructions:

Choose an answer and hit 'next'. You will receive your score and answers at the end.

question 1 of 3

Which of the following ELISAs uses two different antibodies?

I. Direct

II. Sandwich

III. Competitive

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1. In general, what step follows blocking when performing and ELISA?

2. Incubating a plate with antigen or antibody is known as _____.

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About This Quiz & Worksheet

Make your way through the quiz and worksheet to assess your knowledge of enzyme-linked immunosorbent assay. The name for incubating a plate with an antigen or antibody and the function of the substrate in an ELISA are topics on the quiz.

Quiz & Worksheet Goals

You'll be assessed on:

  • Type of ELISA that binds antibody to the plate during the coating step
  • Function of the substrate in an ELISA
  • ELISA that uses two different antibodies
  • Step that follows blocking when performing an ELISA

Skills Practiced

  • Information recall - access the knowledge you have gained about the process of incubating a plate with an antigen or antibody
  • Reading comprehension - ensure that you draw the most important details from the lesson on enzyme-linked immunosorbent assay
  • Knowledge application - use your knowledge to answer questions about different ELISAs

Additional Learning

Take your time looking through the accompanying lesson named Enzyme-Linked Immunosorbent Assay: Definition & Application. Doing so can help you:

  • Define immunosorbent
  • Determine why ELISAs are important
  • Compare and contrast different ELISAs
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