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Lowry Protein Assay: Principle, Protocol & Mechanism

Instructions:

Choose an answer and hit 'next'. You will receive your score and answers at the end.

question 1 of 3

At expected low protein concentrations, what wavelength should be used to measure the protein concentration with the Lowry reaction?

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1. What does the copper sulfate react with in the Lowry protein assay?

2. What compound forms the final color (after the Folins reagent is added) in the Lowry protein assay?

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About This Quiz & Worksheet

Use this multiple-choice quiz and worksheet to find out how much you know about the Lowry protein assay, such as what's involved in a copper sulfate reaction. The quiz can be taken at any time.

Quiz & Worksheet Goals

You'll be tested on the following aspects of the Lowry protein assay:

  • The wavelength used to measure protein concentrations
  • Reaction of the copper sulfate
  • The compound that forms the final color
  • Color change observed when proteins are present
  • Which part of the peptide bonds participate in the Lowry reaction

Skills Practiced

  • Making connections - use what you've learned to make connections between wavelengths and the measuring of protein concentrations
  • Information recall - access the knowledge you've gained about color change in the Lowry protein assay when there are proteins present
  • Interpreting information - verify that you can read information about the part of the peptide bonds that participate in the Lowry protein reaction and interpret it correctly

Additional Learning

Dig deeper into this topic by reviewing the lesson titled Lowry Protein Assay: Principle, Protocol & Mechanism. Here's the type of information you'll find in this lesson:

  • Formation of a monovalent copper ion
  • Use of Bovine Serum Albumin
  • Reduction of copper by the Folins reagent
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