Polymerase Chain Reaction: Definition & Steps


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What is the procedure carried out in a laboratory that allows a scientist to make millions of copies of a piece of DNA?

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1. All of the following are steps in polymerase chain reaction EXCEPT:

2. What is the function of the PCR buffer?

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About This Quiz & Worksheet

Take a look at this multiple-choice quiz/ worksheet to assess your comprehension of the steps in a polymerase chain reaction. Quiz topics include the process completed in a laboratory that permits a scientist to make millions of copies of a piece of DNA and the steps in a chain reaction.

Quiz & Worksheet Goals

You can see what you know about the following by taking the quiz:

  • Role of the PCR buffer
  • Step of PCR when Taq polymerase is utilized
  • The step when a heated test tube allows the DNA double helix to unwind

Skills Practiced

  • Reading comprehension - make sure you know the most important information from the lesson on the steps in a polymerase chain reaction, including the purpose of the PCR buffer
  • Information recall - access the knowledge you've gained regarding the term for the process that's done in a laboratory that allows a scientist to make millions of copies of a piece of DNA
  • Knowledge application - use your knowledge to answer questions about the steps in a PCR and the specific step when Taq polymerase is utilized

Additional Learning

For more on chain reactions, you can review the lesson named Polymerase Chain Reaction: Definition & Steps. These topics will be explored for your benefit:

  • Nucleotides in DNA
  • Addition of a PCR primer
  • PCR cycling to create DNA copies