How to Use Spectrophotometry to Understand Beer's Law


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question 1 of 3

What is the molar absorptivity of the following compound as measured in a 1 cm cuvette?

Concentration: 25uM

Absorption: 0.5874

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1. What is Beer's Law?

2. If I know that the molar absorptivity of a compound measured at 350 nm is 1458/M*cm, can I use 1458 as the molar absorptivity when measuring compounds at 400 nm? Why or why not?

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About This Quiz & Worksheet

This printer-friendly worksheet and interactive quiz will test how much you know about using spectrophotometry to understand Beer's Law. Questions will address main areas of study including the molar absorptivity of a compound with a 25uM concentration and how to find the concentration of a compound in a sample problem.

Quiz & Worksheet Goals

The quiz/worksheet will be covering these topics:

  • Explanation of Beer's Law
  • Whether or not you can use a certain molar absorptivity for different wavelengths
  • Reason the length of a cuvette is important to Beer's Law

Skills Practiced

  • Problem solving - use your acquired knowledge to solve practice problems related to molar absorptivity
  • Defining key concepts - make sure you can accurately define Beer's Law
  • Making connections - use your understanding of the length of a cuvette and how it's connected to Beer's Law

Additional Learning

If you're interested in learning more about these concepts, you can look over the lesson called How to Use Spectrophotometry to Understand Beer's Law. These areas will be covered:

  • Explanation of how a spectrophotometer works
  • What a cuvette is
  • How to use your absorbance results