How to Use Spectrophotometry to Understand Beer's Law

Instructions:

question 1 of 3

What is the molar absorptivity of the following compound as measured in a 1 cm cuvette? Concentration: 25uM Absorption: 0.5874

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2. If I know that the molar absorptivity of a compound measured at 350 nm is 1458/M*cm, can I use 1458 as the molar absorptivity when measuring compounds at 400 nm? Why or why not?

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This printer-friendly worksheet and interactive quiz will test how much you know about using spectrophotometry to understand Beer's Law. Questions will address main areas of study including the molar absorptivity of a compound with a 25uM concentration and how to find the concentration of a compound in a sample problem.

Quiz & Worksheet Goals

The quiz/worksheet will be covering these topics:

• Explanation of Beer's Law
• Whether or not you can use a certain molar absorptivity for different wavelengths
• Reason the length of a cuvette is important to Beer's Law

Skills Practiced

• Problem solving - use your acquired knowledge to solve practice problems related to molar absorptivity
• Defining key concepts - make sure you can accurately define Beer's Law
• Making connections - use your understanding of the length of a cuvette and how it's connected to Beer's Law