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PCR: Steps Involved in Polymerase Chain Reaction

Instructions:

Choose an answer and hit 'next'. You will receive your score and answers at the end.

question 1 of 3

Which of the following represents the correct order of steps in a PCR cycle?

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1. Which of the following statements BEST explains the denaturation step?

2. Which of the following BEST explains the annealing step of PCR?

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About This Quiz & Worksheet

Polymerase chain reactions (PCR) are essential to DNA sequencing as used in forensics today. This quiz will help you evaluate what you've learned by asking questions about the steps in PCR.

Quiz & Worksheet Goals

In this quiz/worksheet combo you will:

  • List the steps in a polymerase chain reaction
  • Explain what happens during specific steps
  • Recognize the purpose of cycling in PCR

Skills Practiced

This quiz and worksheet test the following skills:

  • Reading comprehension - ensure that you draw the most important information from the related polymerase chain reaction lesson
  • Interpreting information - verify that you can read information regarding the steps in a polymerase chain reaction and interpret it correctly
  • Information recall - access the knowledge you've gained regarding the annealing step of PCR

Additional Learning

See the accompanying lesson called PCR: Steps Involved in Polymerase Chain Reaction to learn more about PCR, including:

  • A review of the reagents used in PCR
  • What happens during denaturation, annealing, and extension
  • Why PCR cycling is necessary for template amplification
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