About This Chapter
AEPA General Science: Genetic Engineering - Chapter Summary
This chapter on genetic engineering is designed to get you ready for questions on the AEPA General Science exam. Lesson topics include the function of restriction enzymes, the Sanger Method of DNA sequencing and the use of ligase to engineer recombinant DNA. Other lessons will cover:
- Definition and examples of genetic engineering
- Importance of DNA plasmids in genetic engineering
- Agarose gel electrophoresis
- Ethidium bromide, loading buffers and the DNA ladder
- Bacterial transformation
- Reagents and steps in polymerase chain reactions
Use these informative video lessons to catch up on topics you may not have covered in awhile or to fill in knowledge gaps. The lessons can be viewed as many times as you like until you feel you've mastered the topics. Test your understanding with self-assessment quizzes and the chapter exam.
AEPA General Science: Genetic Engineering Chapter Objectives
The lessons and quizzes in this study guide will get you ready for questions in the life science portion of the test. This test section accounts for about 25% of the total score. Among other topics, you will be asked about methods of genetic engineering and the applications of the process.
The AEPA General Science exam has 150 multiple-choice questions and is administered electronically. You will need to pass the exam if you want to become a science teacher in the state of Arizona.
1. What is Genetic Engineering? - Definition and Examples
How do we make the insulin used by diabetic patients? In this lesson, you'll learn the basics of how genetic engineering can be used to transform a bacterial host cell into a genetically-modified organism that produces human insulin.
2. What is a DNA Plasmid? - Importance to Genetic Engineering
DNA plasmids play an integral part in most genetic engineering experiments. In this lesson, you'll learn about key features of a plasmid, such as a multiple cloning site, an origin of replication, and a selectable marker.
3. Restriction Enzymes: Function and Definition
Restriction enzymes played a critical role in the advent of genetic engineering. In this lesson, you will learn what role restriction enzymes play in creating recombinant DNA.
4. How Ligase is Used to Engineer Recombinant DNA
DNA ligase makes recombinant DNA technology possible. In this lesson, you will learn how new versions of genes can be designed for experiments in novel host organisms using DNA ligase.
5. What is Agarose Gel Electrophoresis?
Agarose gel electrophoresis plays a key role in genetic engineering experiments. In this lesson, you'll learn what agarose is and how electrophoresis works. You'll also discover the use of agarose in this procedure.
6. Ethidium Bromide, Loading Buffer & DNA Ladder: Visualizing DNA and Determining its Size
In this lesson, you will learn about the role that ethidium bromide, loading buffers, and DNA ladders play in visualizing DNA and determining the size of DNA fragments in agarose gel electrophoresis.
7. Agarose Gel Electrophoresis: Equipment & Procedure
This lesson will review the concepts and mechanisms of agarose gel electrophoresis. It will also summarize the equipment needed to perform the procedure for DNA analysis.
8. Agarose Gel Electrophoresis: Results Analysis
Gel electrophoresis is used to analyze DNA restriction digest and ligation experiments. In this lesson, you will learn how to use a DNA ladder to interpret experimental results.
9. Bacterial Transformation: Definition, Process and Genetic Engineering of E. coli
How can a plasmid be inserted into a bacterial cell? How can transformed bacteria carrying a recombinant plasmid be distinguished from untransformed counterparts? These questions and more will be answered in this lesson.
10. Bacterial Transformation: Antibiotic Selection and Positive & Negative Controls
The use of antibiotic selection and positive and negative controls are important elements of interpreting data from a bacterial transformation. In this lesson, you will learn how antibiotic selection results in colony formation and how controls help pinpoint the cause of experimental problems.
11. PCR: Reagents Used in Polymerase Chain Reaction
Polymerase chain reaction (PCR) is a biotechnology technique that is used to amplify pieces of DNA. In this lesson, you will learn about five ingredients necessary to perform PCR: template DNA, nucleotides, primers, buffer and Taq polymerase.
12. PCR: Steps Involved in Polymerase Chain Reaction
In this lesson, you will learn about the steps required to amplify DNA during PCR. The lesson will explain the role template DNA, primers, nucleotides, Taq polymerase and PCR buffer play in the denaturation, annealing and extension steps of PCR.
13. The Sanger Method of DNA Sequencing
The ability to determine the DNA sequence of an individual is a powerful tool for paternity questions and criminal investigations, among other uses. This lesson will describe one laboratory method that can be used to sequence DNA.
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Other chapters within the AEPA General Science (NT311): Practice & Study Guide course
- General Science: Scientific Inquiry
- General Science: Scientific Laws & Theories
- General Science: Scientific Data & Analysis
- General Science: Laboratory Equipment & Safety
- General Science: Science & Society
- General Science: Science in Daily Life
- General Science: Organization of Matter
- General Science: Characteristics of Matter
- General Science: Atomic Structure
- General Science: Nuclear Chemistry
- General Science: Energy & Matter
- General Science: Simple Compounds & Chemical Formulas
- General Science: Types of Chemical Reactions
- General Science: The Mole Concept
- General Science: The Periodic Table
- General Science: Chemical Reactions
- General Science: Equilibrium
- General Science: Acid-Base Chemistry
- General Science: Solutions & Solubility Factors
- General Science: Physics & Motion
- General Science: Newton's Laws
- General Science: Analyzing Motion
- General Science: Analyzing Forces
- General Science: Electrical Concepts
- General Science: Magnetic Fields & Forces
- General Science: Electromagnetic Spectrum
- General Science: Waves, Optics & Sound
- General Science: Cell Structure & Function
- General Science: Cell Reproduction & Division
- General Science: Biochemistry of Life
- General Science: Characteristics of Living Organisms
- General Science: Plant Structures & Functions
- General Science: The Circulatory System
- General Science: The Nervous System
- General Science: The Immune & Endocrine Systems
- General Science: Other Physiological Systems
- General Science: Reproduction & Development
- General Science: Basic Genetics
- General Science: DNA Transcription & Translation Process
- General Science: Evolution
- General Science: History of Life
- General Science: Ecosystems
- General Science: Community & Population Ecology
- General Science: Earth Materials
- General Science: Rocks & Minerals
- General Science: Historical Geology
- General Science: Erosion, Weathering & Wasting
- General Science: Earth's Structure & Processes
- General Science: Plate Tectonics
- General Science: The Water Cycle
- General Science: Oceans
- General Science: Glaciers
- General Science: Other Bodies of Water
- General Science: Earth's Atmosphere & Climate
- General Science: Basic Weather Concepts
- General Science: The Solar System
- General Science: Earth, Moon & Sun
- General Science: Characteristics of the Moon
- General Science: The Universe
- General Science: Orbits of Celestial Bodies
- AEPA General Science Flashcards