Ch 25: Laboratory Techniques in Molecular Biology: Help & Review

About This Chapter

The Basic Molecular Biology Laboratory Techniques chapter of this AP Biology Help and Review course is the simplest way to master biology lab techniques. This chapter uses simple and fun videos that are about five minutes long, plus lesson quizzes and a chapter exam to ensure students learn the essentials of basic molecular biology laboratory techniques.

Who's it for?

Anyone who needs help learning or mastering AP Biology material will benefit from taking this course. There is no faster or easier way to learn AP Biology. Among those who would benefit are:

  • Students who have fallen behind in understanding genetic engineering and molecular biology laboratory techniques
  • Students who struggle with learning disabilities or learning differences, including autism and ADHD
  • Students who prefer multiple ways of learning science (visual or auditory)
  • Students who have missed class time and need to catch up
  • Students who need an efficient way to learn about molecular biology laboratory techniques
  • Students who struggle to understand their teachers
  • Students who attend schools without extra science learning resources

How it works:

  • Find videos in our course that cover what you need to learn or review.
  • Press play and watch the video lesson.
  • Refer to the video transcripts to reinforce your learning.
  • Test your understanding of each lesson with short quizzes.
  • Verify you're ready by completing the molecular biology laboratory techniques chapter exam.

Why it works:

  • Study Efficiently: Skip what you know, review what you don't.
  • Retain What You Learn: Engaging animations and real-life examples make topics easy to grasp.
  • Be Ready on Test Day: Use the molecular biology laboratory techniques chapter exam to be prepared.
  • Get Extra Support: Ask our subject-matter experts any molecular biology laboratory techniques question. They're here to help!
  • Study With Flexibility: Watch videos on any web-ready device.

Students will review:

This chapter helps students review the concepts in a molecular biology laboratory techniques unit of a standard AP Biology course. Topics covered include:

  • DNA plasmid
  • Agarose gel electrophoresis
  • Bacterial transformation
  • Polymerase chain reaction (PCR)
  • Sanger method for sequencing DNA

14 Lessons in Chapter 25: Laboratory Techniques in Molecular Biology: Help & Review
Test your knowledge with a 30-question chapter practice test
What is Genetic Engineering? - Definition and Examples

1. What is Genetic Engineering? - Definition and Examples

Genetic engineering involves modifying a host organism's genetic makeup by using vectors as a transfer agent. Understand the definition and examples of the process, as well as the difference between transgenic and genetically modified organisms.

What is a DNA Plasmid? - Importance to Genetic Engineering

2. What is a DNA Plasmid? - Importance to Genetic Engineering

DNA plasmid is a vital component of genetic engineering. Learn about DNA plasmids and the three characteristics, including multiple cloning sites, replication origin, and selectable markers, that make DNA plasmids so important in genetic engineering.

Restriction Enzymes: Function and Definition

3. Restriction Enzymes: Function and Definition

Understand how restriction enzymes function. Learn about genetic engineering, the definition of a restriction enzyme, the recognition sequence, the palindromic sequence, and the sticky ends of single-strand DNA.

How Ligase is Used to Engineer Recombinant DNA

4. How Ligase is Used to Engineer Recombinant DNA

In relation to recombinant DNA engineering, DNA ligase conjoins two DNA molecules while restriction enzymes cut DNA molecules. Explore how DNA ligase and restriction enzymes work together to facilitate recombinant DNA technology.

What is Agarose Gel Electrophoresis?

5. What is Agarose Gel Electrophoresis?

Gel electrophoresis uses an electrical current to separate biological molecules based on size. Discover concepts like genetic engineering, gel electrophoresis, and agarose.

Ethidium Bromide, Loading Buffer & DNA Ladder: Visualizing DNA and Determining its Size

6. Ethidium Bromide, Loading Buffer & DNA Ladder: Visualizing DNA and Determining its Size

Ethidium bromide, loading buffers, and DNA ladders play a key role in visualizing DNA and determining its size. Explore the importance of these elements in agarose gel electrophoresis - a method that separates biological molecules using electricity.

Agarose Gel Electrophoresis: Equipment & Procedure

7. Agarose Gel Electrophoresis: Equipment & Procedure

Agarose gel electrophoresis is a laboratory procedure that uses an electrical current to separate biological molecules. Explore this procedure and learn about the equipment required to perform it, including a gel box, power supply, loading buffer, dye front, ethidium bromide, and UV box.

Agarose Gel Electrophoresis: Results Analysis

8. Agarose Gel Electrophoresis: Results Analysis

Learn to analyze and interpret the results of an agarose gel electrophoresis. Explore how this lab procedure is used to analyze and troubleshoot the results of restriction digestion or a ligation step in an experiment.

Bacterial Transformation: Definition, Process and Genetic Engineering of E. coli

9. Bacterial Transformation: Definition, Process and Genetic Engineering of E. coli

Bacterial transformation is the process of changing the genetic makeup of a cell by introducing DNA from its surrounding environment. Explore the roles heat shock, recovery, and antibiotic selection play in the process of genetic engineering in this video.

Bacterial Transformation: Antibiotic Selection and Positive & Negative Controls

10. Bacterial Transformation: Antibiotic Selection and Positive & Negative Controls

Scientists conduct carefully designed experiments to form hypotheses and replicate their findings in order to verify them. Learn about bacterial transformation and discover how it uses antibiotic selection and positive and negative controls to identify bacteria that can produce repeatable results.

PCR: Reagents Used in Polymerase Chain Reaction

11. PCR: Reagents Used in Polymerase Chain Reaction

Polymerase chain reaction (PCR) is a procedure that creates copies of DNA using reagents. DNA is amplified, and the amplified DNA regions are identified by other DNA pieces using reagents, such as template DNA and PCR primers, and other key parts of DNA, including nucleotides and tag polymerases. Learn about that process, including the different elements that makes conditions optimal for PCR to occur.

PCR: Steps Involved in Polymerase Chain Reaction

12. PCR: Steps Involved in Polymerase Chain Reaction

Polymerase chain reaction (PCR) is a laboratory procedure that can create replicas of DNA. Explore the three steps of this revolutionary process: denaturation, annealing, and extension.

The Sanger Method of DNA Sequencing

13. The Sanger Method of DNA Sequencing

The Sanger Method of DNA sequencing uses dideoxynucleotides to determine DNA sequencing. Explore the dideoxynucleotide structure, chain terminators, and how to interpret Sanger sequencing in this lesson.

Monomers: Types & Examples

14. Monomers: Types & Examples

Monomers are small molecules able to bond together to form more complex molecules. Discover more about the types of organic monomers and examples including carbohydrates, lipids, nucleic acids, and proteins.

Chapter Practice Exam
Test your knowledge of this chapter with a 30 question practice chapter exam.
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Practice Final Exam
Test your knowledge of the entire course with a 50 question practice final exam.
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