Ch 25: AP Biology: Basic Molecular Biology Laboratory Techniques

About This Chapter

Watch basic molecular biology video lessons and learn about important laboratory techniques, genetic engineering, DNA sequencing and more. These lessons are just a portion of our AP Biology course.

Basic Molecular Biology Laboratory Techniques - Chapter Summary and Learning Objectives

The lessons in this chapter begin with a definition of genetic engineering. You then can learn about DNA plasmid and its relation to genetic engineering. You'll also study more advanced topics like the functions of restriction enzymes and ligase. Lessons cover the process of agarose gel electrophoresis and how to analyze its results. You'll also be introduced to the processes of bacterial transformation and polymerase chain reactions. The chapter closes with an examination of the Sanger method of DNA sequencing. After completing this chapter, you should be able to:

  • Understand genetic engineering
  • Identify the function of restriction enzymes
  • Explain the procedure of agarose gel electrophoresis
  • Define the process of bacterial transformation
  • Name the steps and products used in a polymerase chain reaction
  • Analyze the Sanger method of DNA sequencing

Video Objective
What Is Genetic Engineering? - Definition and Examples Provide a definition and multiple examples of genetic engineering.
What Is a DNA Plasmid? - Importance to Genetic Engineering Explain the importance of DNA plasmids to the process of genetic engineering.
Restriction Enzymes: Function and Definition Recognize the function of restriction enzymes and how they relate to molecular biology.
How Ligase Is Used to Engineer Recombinant DNA Understand how to use ligase to engineer recombinant DNA.
What Is Agarose Gel Electrophoresis? Give a definition of agarose gel electrophoresis.
Ethidium Bromide, Loading Buffer and DNA Ladder: Visualizing DNA and Determining its Size Explain how DNA can be visualized and quantified.
Agarose Gel Electrophoresis: Equipment and Procedure Explain the procedure and equipment needed for agarose gel electrophoresis.
Agarose Gel Electrophoresis: Results Analysis Analyze the results of agarose gel electrophoresis.
Bacterial Transformation: Definition, Process and Genetic Engineering of E. coli Understand bacterial transformation and how it relates to the genetic engineering of E. coli.
Bacterial Transformation: Antibiotic Selection and Positive and Negative Controls Recognize the positive and negative controls used in antibiotic selection.
PCR: Reagents Used in Polymerase Chain Reaction Name the reagents used in polymerase chain reactions.
PCR: Steps Involved in Polymerase Chain Reaction List the steps needed for polymerase chain reactions.
The Sanger Method of DNA Sequencing Define the Sanger method of DNA sequencing.

Chapter Practice Exam
Test your knowledge of this chapter with a 30 question practice chapter exam.
Not Taken
Practice Final Exam
Test your knowledge of the entire course with a 50 question practice final exam.
Not Taken

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