About This Chapter
Who's it for?
This unit of our AP Biology Homeschool course will benefit any student who is trying to learn about the equipment and procedures that are used in the molecular biology laboratory. There is no faster or easier way to learn about biology. Among those who would benefit are:
- Students who require an efficient, self-paced course of study to learn about recombinant DNA, selectable markers, palindromic sequences and electrophoresis.
- Homeschool parents looking to spend less time preparing lessons and more time teaching.
- Homeschool parents who need a biology curriculum that appeals to multiple learning types (visual or auditory).
- Gifted students and students with learning differences.
How it works:
- Students watch a short, fun video lesson that covers a specific unit topic.
- Students and parents can refer to the video transcripts to reinforce learning.
- Short quizzes and a basic molecular biology laboratory techniques unit exam confirm understanding or identify any topics that require review.
Basic Molecular Biology Laboratory Techniques Unit Objectives:
- Learn the definition of genetic engineering.
- Examine the role of the DNA plasmid.
- Study restriction enzymes and recognition sequences.
- Discuss DNA ligase in genetic engineering and replication.
- Discover how agarose gel electrophoresis is used in experiments.
- Explain the functions of ethidium bromide and loading buffers.
- Learn about the equipment that is used in gel electrophoresis.
- Discover how the results of experiments are interpreted with gel electrophoresis.
- Learn about bacterial transformation screening.
- Discuss what happens during a polymerase chain reaction.
- Describe the steps of a polymerase chain reaction.
- Learn about the Sanger sequencing method.
1. What is Genetic Engineering? - Definition and Examples
How do we make the insulin used by diabetic patients? In this lesson, you'll learn the basics of how genetic engineering can be used to transform a bacterial host cell into a genetically-modified organism that produces human insulin.
2. What is a DNA Plasmid? - Importance to Genetic Engineering
DNA plasmids play an integral part in most genetic engineering experiments. In this lesson, you'll learn about key features of a plasmid, such as a multiple cloning site, an origin of replication, and a selectable marker.
3. Restriction Enzymes: Function and Definition
Restriction enzymes played a critical role in the advent of genetic engineering. In this lesson, you will learn what role restriction enzymes play in creating recombinant DNA.
4. How Ligase is Used to Engineer Recombinant DNA
DNA ligase makes recombinant DNA technology possible. In this lesson, you will learn how new versions of genes can be designed for experiments in novel host organisms using DNA ligase.
5. What is Agarose Gel Electrophoresis?
Agarose gel electrophoresis plays a key role in genetic engineering experiments. In this lesson, you'll learn what agarose is and how electrophoresis works. You'll also discover the use of agarose in this procedure.
6. Ethidium Bromide, Loading Buffer & DNA Ladder: Visualizing DNA and Determining its Size
In this lesson, you will learn about the role that ethidium bromide, loading buffers, and DNA ladders play in visualizing DNA and determining the size of DNA fragments in agarose gel electrophoresis.
7. Agarose Gel Electrophoresis: Equipment & Procedure
This lesson will review the concepts and mechanisms of agarose gel electrophoresis. It will also summarize the equipment needed to perform the procedure for DNA analysis.
8. Agarose Gel Electrophoresis: Results Analysis
Gel electrophoresis is used to analyze DNA restriction digest and ligation experiments. In this lesson, you will learn how to use a DNA ladder to interpret experimental results.
9. Bacterial Transformation: Definition, Process and Genetic Engineering of E. coli
How can a plasmid be inserted into a bacterial cell? How can transformed bacteria carrying a recombinant plasmid be distinguished from untransformed counterparts? These questions and more will be answered in this lesson.
10. Bacterial Transformation: Antibiotic Selection and Positive & Negative Controls
The use of antibiotic selection and positive and negative controls are important elements of interpreting data from a bacterial transformation. In this lesson, you will learn how antibiotic selection results in colony formation and how controls help pinpoint the cause of experimental problems.
11. PCR: Reagents Used in Polymerase Chain Reaction
Polymerase chain reaction (PCR) is a biotechnology technique that is used to amplify pieces of DNA. In this lesson, you will learn about five ingredients necessary to perform PCR: template DNA, nucleotides, primers, buffer and Taq polymerase.
12. PCR: Steps Involved in Polymerase Chain Reaction
In this lesson, you will learn about the steps required to amplify DNA during PCR. The lesson will explain the role template DNA, primers, nucleotides, Taq polymerase and PCR buffer play in the denaturation, annealing and extension steps of PCR.
13. The Sanger Method of DNA Sequencing
The ability to determine the DNA sequence of an individual is a powerful tool for paternity questions and criminal investigations, among other uses. This lesson will describe one laboratory method that can be used to sequence DNA.
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Other chapters within the AP Biology: Homeschool Curriculum course
- AP Biology - Science Basics: Homeschool Curriculum
- AP Biology - The Origin of Life on Earth: Homeschool Curriculum
- AP Biology - Evolution Overview: Homeschool Curriculum
- AP Biology - Inorganic Chemistry Review: Homeschool Curriculum
- AP Biology - Organic Chemistry Review: Homeschool Curriculum
- AP Biology - Enzymatic Biochemistry: Homeschool Curriculum
- AP Biology - Cell Biology: Homeschool Curriculum
- AP Biology - Biological Systems Requirements - Homeschool Curriculum
- AP Biology - Cell Division: Homeschool Curriculum
- AP Biology - Metabolic Biochemistry: Homeschool Curriculum
- AP Biology - DNA and RNA Overview: Homeschool Curriculum
- AP Biology - DNA Replication: Homeschool Curriculum
- AP Biology - Transcription & Translation Process: Homeschool Curriculum
- AP Biology - Genetics and Heredity: Homeschool Curriculum
- AP Biology - Genetic Mutations: Homeschool Curriculum
- AP Biology - Phylogeny & Classification of Organisms: Homeschool Curriculum
- AP Biology - Plant Biology: Homeschool Curriculum
- AP Biology - Plant Reproduction & Growth: Homeschool Curriculum
- AP Biology - Animal Reproduction and Development: Homeschool Curriculum
- AP Biology - Reproductive Systems Anatomy & Physiology: Homeschool Curriculum
- AP Biology - Human Body Systems: Homeschool Curriculum
- AP Biology - The Nervous, Immune & Endocrine Systems: Homeschool Curriculum
- AP Biology - Ecology Overview: Homeschool Curriculum
- AP Biology - Animal Behavior: Homeschool Curriculum
- AP Biology - Analyzing Scientific Data: Homeschool Curriculum
- AP Biology - Laboratory: Homeschool Curriculum