About This Chapter
Below is a sample breakdown of the Basic Molecular Biology Laboratory Techniques chapter into a 5-day school week. Based on the pace of your course, you may need to adapt the lesson plan to fit your needs.
|Day||Topics||Key Terms and Concepts Covered|
|Monday|| What is Genetic Engineering? - Definition and Examples; |
What is DNA Plasmid? - Importance to Genetic Engineering;
Restriction Enzymes: Function and Definition
| The purpose of genetic engineering; |
A look at how plasmids are utilized in laboratory environments;
Restriction enzymes and their role in genetic engineering
|Tuesday|| How Ligase is Used to Engineer Recombinant DNA; |
What is Agarose Gel Electrophoresis?;
Ethidium Bromide, Loading Buffer and DNA Ladder: Visualizing DNA and Determining its Size
| The function and necessity of ligase; |
An exploration of the ways that agarose is utilized in genetic engineering;
A look at how ethidium bromide works to tag and visualize DNA molecules
|Wednesday|| Agarose Gel Electrophoresis: Equipment and Procedure; |
Agarose Gel Electrophoresis: Results Analysis;
Bacterial Transformation: Definition, Process and Genetic Engineering
| An overview of this laboratory procedure; |
The process of analyzing and interpreting the results of DNA experiments;
A look at how the process of transformation occurs
|Thursday|| Bacterial Transformation: Antibiotic Selection and Positive and Negative Controls; |
PCR: Reagents Used in Polymerase Chain Reaction
| The functions of bacterial colonies; |
The five ingredients that are needed for this process
|Friday|| PCR: Steps Involved in Polymerase Chain Reaction; |
The Sanger Method of DNA Sequencing
| An explanation of the denaturation, annealing and elongation steps; |
The purposes for applying this method
1. What is Genetic Engineering? - Definition and Examples
How do we make the insulin used by diabetic patients? In this lesson, you'll learn the basics of how genetic engineering can be used to transform a bacterial host cell into a genetically-modified organism that produces human insulin.
2. What is a DNA Plasmid? - Importance to Genetic Engineering
DNA plasmids play an integral part in most genetic engineering experiments. In this lesson, you'll learn about key features of a plasmid, such as a multiple cloning site, an origin of replication, and a selectable marker.
3. Restriction Enzymes: Function and Definition
Restriction enzymes played a critical role in the advent of genetic engineering. In this lesson, you will learn what role restriction enzymes play in creating recombinant DNA.
4. How Ligase is Used to Engineer Recombinant DNA
DNA ligase makes recombinant DNA technology possible. In this lesson, you will learn how new versions of genes can be designed for experiments in novel host organisms using DNA ligase.
5. What is Agarose Gel Electrophoresis?
Agarose gel electrophoresis plays a key role in genetic engineering experiments. In this lesson, you'll learn what agarose is and how electrophoresis works. You'll also discover the use of agarose in this procedure.
6. Ethidium Bromide, Loading Buffer & DNA Ladder: Visualizing DNA and Determining its Size
In this lesson, you will learn about the role that ethidium bromide, loading buffers, and DNA ladders play in visualizing DNA and determining the size of DNA fragments in agarose gel electrophoresis.
7. Agarose Gel Electrophoresis: Equipment & Procedure
This lesson will review the concepts and mechanisms of agarose gel electrophoresis. It will also summarize the equipment needed to perform the procedure for DNA analysis.
8. Agarose Gel Electrophoresis: Results Analysis
Gel electrophoresis is used to analyze DNA restriction digest and ligation experiments. In this lesson, you will learn how to use a DNA ladder to interpret experimental results.
9. Bacterial Transformation: Definition, Process and Genetic Engineering of E. coli
How can a plasmid be inserted into a bacterial cell? How can transformed bacteria carrying a recombinant plasmid be distinguished from untransformed counterparts? These questions and more will be answered in this lesson.
10. Bacterial Transformation: Antibiotic Selection and Positive & Negative Controls
The use of antibiotic selection and positive and negative controls are important elements of interpreting data from a bacterial transformation. In this lesson, you will learn how antibiotic selection results in colony formation and how controls help pinpoint the cause of experimental problems.
11. PCR: Reagents Used in Polymerase Chain Reaction
Polymerase chain reaction (PCR) is a biotechnology technique that is used to amplify pieces of DNA. In this lesson, you will learn about five ingredients necessary to perform PCR: template DNA, nucleotides, primers, buffer and Taq polymerase.
12. PCR: Steps Involved in Polymerase Chain Reaction
In this lesson, you will learn about the steps required to amplify DNA during PCR. The lesson will explain the role template DNA, primers, nucleotides, Taq polymerase and PCR buffer play in the denaturation, annealing and extension steps of PCR.
13. The Sanger Method of DNA Sequencing
The ability to determine the DNA sequence of an individual is a powerful tool for paternity questions and criminal investigations, among other uses. This lesson will describe one laboratory method that can be used to sequence DNA.
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