Ch 35: Basic Molecular Biology Laboratory Techniques: Tutoring Solution

About This Chapter

The Basic Molecular Biology Laboratory Techniques chapter of this High School Biology Tutoring Solution is a flexible and affordable path to learning about basic molecular biology laboratory techniques. These simple and fun video lessons are each about five minutes long and they teach all the processes and steps for molecular biology labs required in a typical high school biology course.

How it works:

  • Begin your assignment or other high school biology work.
  • Identify the basic molecular biology laboratory techniques that you're stuck on.
  • Find fun videos on the topics you need to understand.
  • Press play, watch and learn!
  • Complete the quizzes to test your understanding.
  • As needed, submit a question to one of our instructors for personalized support.

Who's it for?

This chapter of our high school biology tutoring solution will benefit any student who is trying to learn basic molecular biology laboratory techniques and earn better grades. This resource can help students including those who:

  • Struggle with understanding genetic engineering, restriction enzyme function, bacterial transformations, reagents or any other basic molecular biology laboratory topic.
  • Have limited time for studying
  • Want a cost effective way to supplement their science learning
  • Prefer learning science visually
  • Find themselves failing or close to failing their basic molecular biology laboratory techniques unit
  • Cope with ADD or ADHD
  • Want to get ahead in high school biology
  • Don't have access to their science teacher outside of class

Why it works:

  • Engaging Tutors: We make learning about basic molecular biology laboratory techniques simple and fun.
  • Cost Efficient: For less than 20% of the cost of a private tutor, you'll have unlimited access 24/7.
  • Consistent High Quality: Unlike a live biology tutor, these video lessons are thoroughly reviewed.
  • Convenient: Imagine a tutor as portable as your laptop, tablet or smartphone. Learn about basic molecular biology laboratory techniques on the go!
  • Learn at Your Pace: You can pause and rewatch lessons as often as you'd like, until you master the material.

Learning Objectives

  • Define genetic engineering.
  • Explain the importance of the DNA plasmid in genetic engineering.
  • Describe the function of restriction enzymes.
  • Learn how recombinant DNA can be engineered using ligase.
  • Explain the purpose of agarose gel electrophoresis.
  • Describe the methods by which scientists visualize DNA and determine its size.
  • List the equipment used for agarose gel electrophoresis and understand how the procedure is performed.
  • Explain how the results of agarose gel electrophoresis are analyzed.
  • Discuss the process of bacterial transformation and describe how E. coli is genetically engineered.
  • Learn the significance of antibiotic selection and positive and negative controls in interpreting bacterial transformation data.
  • Name the reagents used in polymerase chain reactions.
  • List the steps of a polymerase chain reaction.
  • Understand the Sanger method for DNA sequencing.

15 Lessons in Chapter 35: Basic Molecular Biology Laboratory Techniques: Tutoring Solution
Test your knowledge with a 30-question chapter practice test
What is Genetic Engineering? - Definition and Examples

1. What is Genetic Engineering? - Definition and Examples

How do we make the insulin used by diabetic patients? In this lesson, you'll learn the basics of how genetic engineering can be used to transform a bacterial host cell into a genetically-modified organism that produces human insulin.

What is a DNA Plasmid? - Importance to Genetic Engineering

2. What is a DNA Plasmid? - Importance to Genetic Engineering

DNA plasmids play an integral part in most genetic engineering experiments. In this lesson, you'll learn about key features of a plasmid, such as a multiple cloning site, an origin of replication, and a selectable marker.

Restriction Enzymes: Function and Definition

3. Restriction Enzymes: Function and Definition

Restriction enzymes played a critical role in the advent of genetic engineering. In this lesson, you will learn what role restriction enzymes play in creating recombinant DNA.

How Ligase is Used to Engineer Recombinant DNA

4. How Ligase is Used to Engineer Recombinant DNA

DNA ligase makes recombinant DNA technology possible. In this lesson, you will learn how new versions of genes can be designed for experiments in novel host organisms using DNA ligase.

What is Agarose Gel Electrophoresis?

5. What is Agarose Gel Electrophoresis?

Agarose gel electrophoresis plays a key role in genetic engineering experiments. In this lesson, you'll learn what agarose is and how electrophoresis works. You'll also discover the use of agarose in this procedure.

Ethidium Bromide, Loading Buffer & DNA Ladder: Visualizing DNA and Determining its Size

6. Ethidium Bromide, Loading Buffer & DNA Ladder: Visualizing DNA and Determining its Size

In this lesson, you will learn about the role that ethidium bromide, loading buffers, and DNA ladders play in visualizing DNA and determining the size of DNA fragments in agarose gel electrophoresis.

Agarose Gel Electrophoresis: Equipment & Procedure

7. Agarose Gel Electrophoresis: Equipment & Procedure

This lesson will review the concepts and mechanisms of agarose gel electrophoresis. It will also summarize the equipment needed to perform the procedure for DNA analysis.

Agarose Gel Electrophoresis: Results Analysis

8. Agarose Gel Electrophoresis: Results Analysis

Gel electrophoresis is used to analyze DNA restriction digest and ligation experiments. In this lesson, you will learn how to use a DNA ladder to interpret experimental results.

Bacterial Transformation: Definition, Process and Genetic Engineering of E. coli

9. Bacterial Transformation: Definition, Process and Genetic Engineering of E. coli

How can a plasmid be inserted into a bacterial cell? How can transformed bacteria carrying a recombinant plasmid be distinguished from untransformed counterparts? These questions and more will be answered in this lesson.

Bacterial Transformation: Antibiotic Selection and Positive & Negative Controls

10. Bacterial Transformation: Antibiotic Selection and Positive & Negative Controls

The use of antibiotic selection and positive and negative controls are important elements of interpreting data from a bacterial transformation. In this lesson, you will learn how antibiotic selection results in colony formation and how controls help pinpoint the cause of experimental problems.

PCR: Reagents Used in Polymerase Chain Reaction

11. PCR: Reagents Used in Polymerase Chain Reaction

Polymerase chain reaction (PCR) is a biotechnology technique that is used to amplify pieces of DNA. In this lesson, you will learn about five ingredients necessary to perform PCR: template DNA, nucleotides, primers, buffer and Taq polymerase.

PCR: Steps Involved in Polymerase Chain Reaction

12. PCR: Steps Involved in Polymerase Chain Reaction

In this lesson, you will learn about the steps required to amplify DNA during PCR. The lesson will explain the role template DNA, primers, nucleotides, Taq polymerase and PCR buffer play in the denaturation, annealing and extension steps of PCR.

The Sanger Method of DNA Sequencing

13. The Sanger Method of DNA Sequencing

The ability to determine the DNA sequence of an individual is a powerful tool for paternity questions and criminal investigations, among other uses. This lesson will describe one laboratory method that can be used to sequence DNA.

Feedback Inhibition: Definition & Example

14. Feedback Inhibition: Definition & Example

This lesson will review enzymes, substrates and products, then give a definition of feedback inhibition and discuss how it works. Some examples of feedback inhibition will also be discussed.

Methanogens: Definition, Classification & Examples

15. Methanogens: Definition, Classification & Examples

Tiny organisms that can die if they are exposed to oxygen? Sounds strange, but it's just an ordinary day for a methanogen. This lesson will explore methanogens, describing their distinguishing characteristics and then giving some examples.

Chapter Practice Exam
Test your knowledge of this chapter with a 30 question practice chapter exam.
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Practice Final Exam
Test your knowledge of the entire course with a 50 question practice final exam.
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