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- Become familiar with the conservative, semi-conservative and dispersive models of DNA replication.
- Explain how DNA helicase takes apart the DNA double helix in preparation for replication.
- Describe how DNA replication is initiated by DNA polymerase and RNA primase.
- Explain the role of the leading strand and DNA polymerase activities in DNA replication.
- Discuss how replication bubbles are formed during DNA replication.
1. What Is DNA Replication? - Conservative, Semi-Conservative & Dispersive Models
How do we know that DNA replication is semi-conservative? How do we know it's not conservative or dispersive? Let's follow the famous experiment by Meselson and Stahl to find out!
2. How Helicase Unwinds the DNA Double Helix in Preparation for Replication
How does semi-conservative replication begin? Discover how DNA helicase creates a replication fork to unwind the complicated DNA molecule and allow daughter strands to form on the parental template.
3. How DNA Polymerase and RNA Primase Initiate DNA Replication
How do enzymes assist in starting DNA replication? In this lesson, we explore the work of a contributing enzyme, DNA polymerase, and learn how the RNA primer is made by the action of RNA primase.
4. DNA Replication: The Leading Strand and DNA Polymerase Activities
How does replication occur in the antiparallel DNA molecule? In this lesson, explore the significance of the leading and lagging strands, and learn how Okazaki fragments and RNA ligase make DNA replication possible.
5. DNA Replication: Review of Enzymes, Replication Bubbles & Leading and Lagging Strands
Feeling lost in the thorny details of DNA replication? This lesson provides an overview of semi-conservative replication, with a focus on putting together all of the concepts involved. We'll review the work of each enzyme on our way to completing the big picture of DNA replication.
6. Non-Coding Regions of DNA: Sequences & Explanation
In biology we learn that DNA is the blueprint for life. However, 98% of your DNA does not code for proteins. This non-coding DNA has been called useless, worthless, in a word, 'junk DNA.' But is it really worthless? Read this lesson and find out.
7. Replication Bubble: Definition & Overview
A replication bubble is the setting for DNA replication. In this lesson, we will review DNA, then find out why replication bubbles are called a bubble, what stabilizes them and how DNA polymerase finishes up.
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