About This Chapter
Genetic Manipulation Overview - Chapter Summary
Feel confident in your knowledge of restriction enzymes, bacterial transformation and agarose gel electrophoresis by exploring this genetic manipulation overview. Strengthen your comprehension of these and other related topics by reviewing bite-sized lessons and taking accompanying multiple-choice quizzes. Our Dashboard traces your steps through this chapter and lets you submit any lesson topic questions you have to our subject-matter experts. By utilizing these learning and study resources, you will be ready and able to do the following in a short amount of time:
- Explain how DNA plasmids are used in genetic engineering
- Detail how recombinant DNA is engineered using ligase
- Discuss the roles of ethidium bromide, loading buffers and DNA ladders in visualizing DNA
- Define the polymerase chain reaction
- Describe the process of DNA cloning
- Discuss the importance of studying genomic DNA
- Exhibit knowledge of the practical applications and safety concerns of DNA technology
1. Genetic Engineering & DNA Plasmid
This lesson will discuss what a GMO is, and how DNA plasmids are used in genetic engineering. The lesson will include these important features of plasmids: a multiple cloning site, an origin of replication, and a selectable marker.
2. Restriction Enzymes: Function and Definition
Restriction enzymes played a critical role in the advent of genetic engineering. In this lesson, you will learn what role restriction enzymes play in creating recombinant DNA.
3. How Ligase is Used to Engineer Recombinant DNA
DNA ligase makes recombinant DNA technology possible. In this lesson, you will learn how new versions of genes can be designed for experiments in novel host organisms using DNA ligase.
4. Agarose Gel Electrophoresis: Procedure & Analysis
Gel electrophoresis is a biology lab technique used to analyze large molecules like DNA. The technique relies on moving charged molecules through a gelatin-like matrix using an electric field.
5. Ethidium Bromide, Loading Buffer & DNA Ladder: Visualizing DNA and Determining its Size
In this lesson, you will learn about the role that ethidium bromide, loading buffers, and DNA ladders play in visualizing DNA and determining the size of DNA fragments in agarose gel electrophoresis.
6. Bacterial Transformation: Definition, Steps & Analysis
Bacteria can pick up genes or pieces of DNA from their environment in a process called transformation. In this lesson, we'll explore how this natural process can be exploited in the lab for genetic engineering of bacteria.
7. Polymerase Chain Reaction: Definition & Steps
Have you ever wondered if you could make millions of copies of something in a matter of minutes. We're not discussing a copy machine, but rather a fascinating laboratory procedure called polymerase chain reaction. Learn all about it in this lesson!
8. DNA Cloning: Definition and Process
DNA cloning is used for a variety of purposes, but how does it work? In this video lesson, you will learn about the process of cloning DNA, as well as see examples of how cloning is used in science, medicine, and consumer products.
9. Genomic DNA Analysis
Your genes are what make you who you are, but they also make you a human. Studying genes and genomes allows scientists to better understand not only our own species, but how we are related to other species in the world as well.
10. Practical Applications & Safety Concerns of DNA Technology
Our discovery of DNA was a big deal, but what technologies has it led to? And could they be dangerous? Learn about DNA technology, and then test your knowledge with a quiz.
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Other chapters within the Biology 203: Cell & Molecular Biology course
- Biological Molecules
- Cell Structure & Function Overview
- Cell Membrane & Transport Overview
- Principles of Cell Metabolism
- Photosynthesis Fundamentals
- Cellular Respiration & Fermentation
- Cell Cycles
- Patterns of Genetic Inheritance
- DNA Structure & Replication
- Mutation & DNA Damage
- The Central Dogma of Biology & Protein Synthesis
- Structure & Function of Viruses
- Control of Gene Expression