About This Chapter
ILTS Biology: Genetic Engineering & Cloning - Chapter Summary
Refresh your understanding of what is involved in genetic engineering and how various processes are used. Concepts covered in this chapter will help you tackle ILTS Biology exams on topics that include:
- The DNA plasmid and genetic engineering
- Function of restriction enzymes
- Ligase and its use in engineering recombinant DNA
- Agarose gel electrophoresis
- The processes involved in bacterial transformations and polymerase chain reactions
- DNA cloning and analysis
- Applications of DNA technology
You can watch these lessons on a computer or mobile device as your schedule permits. Our Dashboard feature keeps track of your progress through the lessons. It even has an ask-an-expert message board that lets you pose questions to the instructors. Use the lesson quizzes to assess your learning and take advantage of the links in the video timeline to go back and review specific parts of the lesson.
ILTS Biology: Genetic Engineering & Cloning Chapter Objectives
Illinois uses the ILTS Biology test to assess the understanding of biology fundamentals in its candidates for teacher certification. The exam's questions are all multiple-choice, like the questions on our quizzes. Taking the quizzes will give you real experience answering questions in the format you'll face on the exam. The exam is divided into six subareas, and questions on the topics covered in this Genetic Engineering and Cloning chapter are part of the Cell Biology, Heredity and Evolution subarea.
1. Genetic Engineering & DNA Plasmid
This lesson will discuss what a GMO is, and how DNA plasmids are used in genetic engineering. The lesson will include these important features of plasmids: a multiple cloning site, an origin of replication, and a selectable marker.
2. Restriction Enzymes: Function and Definition
Restriction enzymes played a critical role in the advent of genetic engineering. In this lesson, you will learn what role restriction enzymes play in creating recombinant DNA.
3. How Ligase is Used to Engineer Recombinant DNA
DNA ligase makes recombinant DNA technology possible. In this lesson, you will learn how new versions of genes can be designed for experiments in novel host organisms using DNA ligase.
4. Ethidium Bromide, Loading Buffer & DNA Ladder: Visualizing DNA and Determining its Size
In this lesson, you will learn about the role that ethidium bromide, loading buffers, and DNA ladders play in visualizing DNA and determining the size of DNA fragments in agarose gel electrophoresis.
5. What is Agarose Gel Electrophoresis?
Agarose gel electrophoresis plays a key role in genetic engineering experiments. In this lesson, you'll learn what agarose is and how electrophoresis works. You'll also discover the use of agarose in this procedure.
6. Bacterial Transformation: Definition, Process & Applications
DNA is all around you. So are bacteria. Did you know that those bacteria can pick up and use that DNA? In this lesson we will examine the process of transformation and how bacteria are able to make use of environmental DNA.
7. Polymerase Chain Reaction: Definition & Steps
Have you ever wondered if you could make millions of copies of something in a matter of minutes. We're not discussing a copy machine, but rather a fascinating laboratory procedure called polymerase chain reaction. Learn all about it in this lesson!
8. DNA Cloning: Definition and Process
DNA cloning is used for a variety of purposes, but how does it work? In this video lesson, you will learn about the process of cloning DNA, as well as see examples of how cloning is used in science, medicine, and consumer products.
9. Genomic DNA Analysis
Your genes are what make you who you are, but they also make you a human. Studying genes and genomes allows scientists to better understand not only our own species, but how we are related to other species in the world as well.
10. Practical Applications & Safety Concerns of DNA Technology
Our discovery of DNA was a big deal, but what technologies has it led to? And could they be dangerous? Learn about DNA technology, and then test your knowledge with a quiz.
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