About This Chapter
About this Chapter
Are you ready to get hands-on in the lab? This chapter on laboratory techniques will teach you about how research works, from prepping the lab to measuring bacterial growth. Instructors will also discuss specific procedures and tools, such as methods for the Gram Stain, serial dilutions, and differential and selective media. Upon completing these lessons, students will be prepared to:
- Explain how to prepare a lab to create the correct conditions for growing bacteria
- Describe the phases of bacterial growth and how they are measured
- Define and discuss the Gram Stain and serial dilution
- Compare and contrast differential and selective media and explain how they are used in a lab
|The Gram Stain: Methods, Techniques & Processes||Learn about the Gram Stain procedure, how it differentiates Gram-positive and Gram-negative bacteria, and how it applies to relevant organisms.|
|Growing Bacteria in a Lab: Experiments & Conditions||Discover basic bacteriology procedures, including how to use sterile technique, and get an overview of growth and isolation techniques in the lab.|
|What Is Bacterial Growth & Generation Time? - Curves, Phases & Stages||Explore bacterial growth, characteristics of bacterial growth curves, and generation times, including lag phase and log phase.|
|Serial Dilution in Microbiology: Calculation, Method & Technique||Define serial dilutions and discuss how they are used in microbiology.|
|Differential & Selective Media in Microbiology||Learn about using selective and differential media, how they are different, and how they are used in a microbiology lab, including classification, selecting for bacteria with a certain plasmid, and showing that a bacterial plasmid has a piece of DNA incorporated into it.|
1. Simple and Differential Stains: Definition and Examples
Observing tiny bacteria under the microscope is not as easy as it sounds. In this lesson, we will examine several staining techniques used to color bacteria, enhancing their visibility.
2. The Gram Stain: Background and Example Organisms
Every microbiology student should be familiar with the Gram stain. In this first of two lessons on this technique, we will look at some of the background information before describing and viewing some relevant example organisms.
3. The Gram Stain: Theory and Procedure
In this lesson, we will focus on the individual steps of the Gram stain procedure. In this second of two lessons on the Gram stain, we will examine the theory and procedure behind how each step contributes to the process as a whole.
4. Growing Bacteria in a Lab: Experiments & Conditions
Despite the fact that bacteria are growing all around you, they can be surprisingly difficult to grow in a lab. This lesson will explore some of the basics involved in culturing bacteria for experimentation.
5. What Is Bacterial Growth & Generation Time? - Curves, Phases & Stages
Bacteria are able to reproduce at an incredibly rapid pace. In this lesson, we will examine the different phases of bacterial growth and how to calculate population numbers based on generation times and culture size.
6. Serial Dilution in Microbiology: Calculation, Method & Technique
Working with billions of tiny cells can pose a problem when you need to count the total number of cells in a sample. Fortunately, through precise serial dilution of a sample, it is possible to get down to a number that is much easier to work with.
7. Differential & Selective Media in Microbiology
Every microbiologist eventually has to grow cultures of bacteria for their experiments. In this lesson, we will investigate selective and differential media, two techniques to make culturing bacterial species a little more efficient.
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Other chapters within the Microbiology 101: Intro to Microbiology course
- Biology Review
- Microbiology Basics
- Bacterial Biology
- Microorganisms and the Environment
- The Disease Process
- Protozoan Diseases
- Introduction to Viruses
- DNA Viruses
- RNA Viruses
- Fungal Infections
- Foodborne Illnesses and Bacterial Infections of the Gastrointestinal Tract
- Sexually Transmitted Bacterial Diseases
- Blood-borne Bacterial Diseases
- Bacterial Diseases of the Respiratory Tract
- Bacterial Skin and Wound Infections
- Immunology And the Body's Defenses Against Pathogens
- Antimicrobial Drugs
- Food and Industrial Microbiology
- Sterilization and Antiseptic Techniques