About This Chapter
Who's it for?
This Molecular Biology Lab Techniques unit of our High School Biology Homeschool course will benefit any student who is trying to learn about genetic engineering. There is no faster or easier way to learn about molecular biology lab techniques. Among those who would benefit are:
- Students who require an efficient, self-paced course of study to learn about genetic engineering, DNA plasmid and restriction enzymes.
- Homeschool parents looking to spend less time preparing lessons and more time teaching.
- Homeschool parents who need a biology curriculum that appeals to multiple learning types (visual or auditory).
- Gifted students and students with learning differences.
How it works:
- Students watch a short, fun video lesson that covers a specific unit topic.
- Students and parents can refer to the video transcripts to reinforce learning.
- Short quizzes and the Molecular Biology Lab Techniques unit exam confirm understanding or identify any topics that require review.
Molecular Biology Lab Techniques Unit Objectives:
- Read about agarose gel electrophoresis, including equipment and methodology.
- Discover why ligase is important to the engineering of recombinant DNA.
- Learn about the processes involved in bacterial transformation.
- Explore the different stages found in polymerase chain reactions.
- Read about the DNA ladder.
- Explore the Sanger method of DNA sequencing.
1. What is Genetic Engineering? - Definition and Examples
How do we make the insulin used by diabetic patients? In this lesson, you'll learn the basics of how genetic engineering can be used to transform a bacterial host cell into a genetically-modified organism that produces human insulin.
2. What is a DNA Plasmid? - Importance to Genetic Engineering
DNA plasmids play an integral part in most genetic engineering experiments. In this lesson, you'll learn about key features of a plasmid, such as a multiple cloning site, an origin of replication, and a selectable marker.
3. Restriction Enzymes: Function and Definition
Restriction enzymes played a critical role in the advent of genetic engineering. In this lesson, you will learn what role restriction enzymes play in creating recombinant DNA.
4. How Ligase is Used to Engineer Recombinant DNA
DNA ligase makes recombinant DNA technology possible. In this lesson, you will learn how new versions of genes can be designed for experiments in novel host organisms using DNA ligase.
5. What is Agarose Gel Electrophoresis?
Agarose gel electrophoresis plays a key role in genetic engineering experiments. In this lesson, you'll learn what agarose is and how electrophoresis works. You'll also discover the use of agarose in this procedure.
6. Ethidium Bromide, Loading Buffer & DNA Ladder: Visualizing DNA and Determining its Size
In this lesson, you will learn about the role that ethidium bromide, loading buffers, and DNA ladders play in visualizing DNA and determining the size of DNA fragments in agarose gel electrophoresis.
7. Agarose Gel Electrophoresis: Equipment & Procedure
This lesson will review the concepts and mechanisms of agarose gel electrophoresis. It will also summarize the equipment needed to perform the procedure for DNA analysis.
8. Agarose Gel Electrophoresis: Results Analysis
Gel electrophoresis is used to analyze DNA restriction digest and ligation experiments. In this lesson, you will learn how to use a DNA ladder to interpret experimental results.
9. Bacterial Transformation: Definition, Process and Genetic Engineering of E. coli
How can a plasmid be inserted into a bacterial cell? How can transformed bacteria carrying a recombinant plasmid be distinguished from untransformed counterparts? These questions and more will be answered in this lesson.
10. Bacterial Transformation: Antibiotic Selection and Positive & Negative Controls
The use of antibiotic selection and positive and negative controls are important elements of interpreting data from a bacterial transformation. In this lesson, you will learn how antibiotic selection results in colony formation and how controls help pinpoint the cause of experimental problems.
11. PCR: Reagents Used in Polymerase Chain Reaction
Polymerase chain reaction (PCR) is a biotechnology technique that is used to amplify pieces of DNA. In this lesson, you will learn about five ingredients necessary to perform PCR: template DNA, nucleotides, primers, buffer and Taq polymerase.
12. PCR: Steps Involved in Polymerase Chain Reaction
In this lesson, you will learn about the steps required to amplify DNA during PCR. The lesson will explain the role template DNA, primers, nucleotides, Taq polymerase and PCR buffer play in the denaturation, annealing and extension steps of PCR.
13. The Sanger Method of DNA Sequencing
The ability to determine the DNA sequence of an individual is a powerful tool for paternity questions and criminal investigations, among other uses. This lesson will describe one laboratory method that can be used to sequence DNA.
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Other chapters within the High School Biology: Homeschool Curriculum course
- Science Vocabulary & Basics: Homeschool Currilum
- Science Lab Skills: Homeschool Curriculum
- Inorganic Chemistry Review: Homeschool Curriculum
- Organic Chemistry Introduction: Homeschool Curriculum
- Functions & Reactions of Enzymes: Homeschool Curriculum
- Cell Biology: Homeschool Curriculum
- Requirements of Biological Systems: Homeschool Curriculum
- Cell Communication: Homeschool Curriculum
- Metabolic Biochemistry: Homeschool Curriculum
- Cell Division: Homeschool Curriculum
- Nucleic Acids: Homeschool Curriculum
- DNA Replication: Homeschool Curriculum
- Transcription & Translation: Homeschool Curriculum
- Principles of Heredity: Homeschool Curriculum
- Genetic Mutations: Homeschool Curriculum
- DNA Technology & Genomics: Homeschool Curriculum
- Bacterial Biology: Homeschool Curriculum
- Introduction to Viruses: Homeschool Curriculum
- The Origin of the Universe: Homeschool Curriculum
- Geologic Time: Homeschool Curriculum
- Evolution Overview: Homeschool Curriculum
- The Classification of Organisms: Homeschool Curriculum
- Plant Biology: Homeschool Curriculum
- Plant Reproduction & Growth: Homeschool Curriculum
- Introduction to Fungi: Homeschool Curriculum
- Introduction to Invertebrates: Homeschool Curriculum
- Introduction to Vertebrates: Homeschool Curriculum
- The Circulatory & Respiratory Systems: Homeschool Curriculum
- The Immune & Endocrine Systems: Homeschool Curriculum
- Animal Reproduction & Development: Homeschool Curriculum
- Human Reproductive Systems: Homeschool Curriculum
- Ecology & the Environment: Homeschool Curriculum
- The Environmental Impact of Humans: Homeschool Curriculum
- Animal Behavior: Homeschool Curriculum
- Analyzing Scientific Data: Homeschool Curriculum