About This Chapter
Who's It For?
Anyone who needs help learning or mastering molecular biology laboratory methods material will benefit from the lessons in this chapter. There is no faster or easier way to learn about molecular biology laboratory methods. Among those who would benefit are:
- Students who have fallen behind in understanding the Sanger method of sequencing DNA or the importance of a DNA plasmid
- Students who struggle with learning disabilities or learning differences, including autism and ADHD
- Students who prefer multiple ways of learning biology (visual or auditory)
- Students who have missed class time and need to catch up
- Students who need an efficient way to learn about molecular biology laboratory methods
- Students who struggle to understand their teachers
- Students who attend schools without extra biology learning resources
How It Works:
- Find videos in our course that cover what you need to learn or review.
- Press play and watch the video lesson.
- Refer to the video transcripts to reinforce your learning.
- Test your understanding of each lesson with short quizzes.
- Verify you're ready by completing the Molecular Biology Laboratory Methods chapter exam.
Why It Works:
- Study Efficiently: Skip what you know, review what you don't.
- Retain What You Learn: Engaging animations and real-life examples make topics easy to grasp.
- Be Ready on Test Day: Use the Molecular Biology Laboratory Methods chapter exam to be prepared.
- Get Extra Support: Ask our subject-matter experts any molecular biology laboratory methods question. They're here to help!
- Study With Flexibility: Watch videos on any web-ready device.
Students Will Review:
This chapter helps students review the concepts in a molecular biology laboratory methods unit of a standard molecular biology course. Topics covered include:
- The definition and examples of genetic engineering
- DNA plasmids
- Restriction enzymes
- The use of ligase in engineering recombinant DNA
- The definition of agarose gel electrophoresis
- Ethidium bromide
- The equipment, procedures and results analysis of agarose gel electrophoresis
- The definition and processes of bacterial transformation
- The steps and reagents used in polymerase chain reaction
- The Sanger method
1. What is Genetic Engineering? - Definition and Examples
How do we make the insulin used by diabetic patients? In this lesson, you'll learn the basics of how genetic engineering can be used to transform a bacterial host cell into a genetically-modified organism that produces human insulin.
2. What is a DNA Plasmid? - Importance to Genetic Engineering
DNA plasmids play an integral part in most genetic engineering experiments. In this lesson, you'll learn about key features of a plasmid, such as a multiple cloning site, an origin of replication, and a selectable marker.
3. Restriction Enzymes: Function and Definition
Restriction enzymes played a critical role in the advent of genetic engineering. In this lesson, you will learn what role restriction enzymes play in creating recombinant DNA.
4. How Ligase is Used to Engineer Recombinant DNA
DNA ligase makes recombinant DNA technology possible. In this lesson, you will learn how new versions of genes can be designed for experiments in novel host organisms using DNA ligase.
5. What is Agarose Gel Electrophoresis?
Agarose gel electrophoresis plays a key role in genetic engineering experiments. In this lesson, you'll learn what agarose is and how electrophoresis works. You'll also discover the use of agarose in this procedure.
6. Ethidium Bromide, Loading Buffer & DNA Ladder: Visualizing DNA and Determining its Size
In this lesson, you will learn about the role that ethidium bromide, loading buffers, and DNA ladders play in visualizing DNA and determining the size of DNA fragments in agarose gel electrophoresis.
7. Agarose Gel Electrophoresis: Equipment & Procedure
This lesson will review the concepts and mechanisms of agarose gel electrophoresis. It will also summarize the equipment needed to perform the procedure for DNA analysis.
8. Agarose Gel Electrophoresis: Results Analysis
Gel electrophoresis is used to analyze DNA restriction digest and ligation experiments. In this lesson, you will learn how to use a DNA ladder to interpret experimental results.
9. Bacterial Transformation: Definition, Process and Genetic Engineering of E. coli
How can a plasmid be inserted into a bacterial cell? How can transformed bacteria carrying a recombinant plasmid be distinguished from untransformed counterparts? These questions and more will be answered in this lesson.
10. Bacterial Transformation: Antibiotic Selection and Positive & Negative Controls
The use of antibiotic selection and positive and negative controls are important elements of interpreting data from a bacterial transformation. In this lesson, you will learn how antibiotic selection results in colony formation and how controls help pinpoint the cause of experimental problems.
11. PCR: Reagents Used in Polymerase Chain Reaction
Polymerase chain reaction (PCR) is a biotechnology technique that is used to amplify pieces of DNA. In this lesson, you will learn about five ingredients necessary to perform PCR: template DNA, nucleotides, primers, buffer and Taq polymerase.
12. PCR: Steps Involved in Polymerase Chain Reaction
In this lesson, you will learn about the steps required to amplify DNA during PCR. The lesson will explain the role template DNA, primers, nucleotides, Taq polymerase and PCR buffer play in the denaturation, annealing and extension steps of PCR.
13. The Sanger Method of DNA Sequencing
The ability to determine the DNA sequence of an individual is a powerful tool for paternity questions and criminal investigations, among other uses. This lesson will describe one laboratory method that can be used to sequence DNA.
14. Chromatography in Forensic Science
In this lesson, we'll discuss what chromatography is and why it's useful to forensic scientists. We'll focus on thin-layer chromatography and how it works, but we'll also mention some other types of chromatography.
15. Cell Culture Contamination: Types & Identification
This lesson is going to go over something known as a cell culture. You'll learn what it is, how it may be contaminated, and some of the ways by which this contamination may be spotted.
16. Tyndallization Sterilization: Definition, Process & History
Tyndallization sterilization is a method of sterilization developed by physicist Dr. John Tyndall during the nineteenth century. This lesson will examine Tyndallization, its history, how it works, and its uses and effectiveness.
17. E-Beam Sterilization: Process & Equipment
E-beam sterilization is a technology that uses a beam of electrons to sterilize various equipment. It is a safe and reliable method with useful applications. This lesson will discuss the E-beam sterilization process and some of the equipment used.
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