About This Chapter
NYSTCE Biology: Genetic Engineering & Cloning - Chapter Summary
Use these study tools to get ready for questions about cloning and genetic engineering on the NYSTCE Biology exam. The video lessons in this chapter will get you up to speed on topics like agarose gel electrophoresis, polymerase chain reactions, and the practical applications of DNA technologies. By the end of the chapter, you will also be proficient in the following:
- DNA plasmids and genetic engineering
- Restriction enzymes
- Loading buffers, ethidium bromide and the DNA ladder
- Bacterial transformation
- Process of DNA cloning
- DNA analysis
- The use of ligase in genetic engineering
Our video lessons are fun to watch, and they last between five and ten minutes each. Watch them as many times as you'd like, or use the lesson transcripts to browse the material. The self-assessment quizzes and chapter exam will give you a good idea of the types of questions you'll be asked on the NYSTCE exam.
NYSTCE Biology: Genetic Engineering & Cloning Objectives
The NYSTCE Biology exam is part of the New York state licensure requirements to teach biology in public schools. You can expect to answer 90 selected-response questions and one constructed-response question.
Our expert instructors have designed the lessons in this chapter to help you answer questions about genetic engineering and cloning in the cell biology and biochemistry subarea of the NYSTCE Biology exam. This exam portion makes up about 17% of the total test questions. Among other topics, you'll be asked about the procedures that go into the isolation, expression and manipulation of genetic material. You'll also need to demonstrate your understanding of the applications of genetic engineering.
1. Genetic Engineering & DNA Plasmid
This lesson will discuss what a GMO is, and how DNA plasmids are used in genetic engineering. The lesson will include these important features of plasmids: a multiple cloning site, an origin of replication, and a selectable marker.
2. Restriction Enzymes: Function and Definition
Restriction enzymes played a critical role in the advent of genetic engineering. In this lesson, you will learn what role restriction enzymes play in creating recombinant DNA.
3. How Ligase is Used to Engineer Recombinant DNA
DNA ligase makes recombinant DNA technology possible. In this lesson, you will learn how new versions of genes can be designed for experiments in novel host organisms using DNA ligase.
4. Ethidium Bromide, Loading Buffer & DNA Ladder: Visualizing DNA and Determining its Size
In this lesson, you will learn about the role that ethidium bromide, loading buffers, and DNA ladders play in visualizing DNA and determining the size of DNA fragments in agarose gel electrophoresis.
5. What is Agarose Gel Electrophoresis?
Agarose gel electrophoresis plays a key role in genetic engineering experiments. In this lesson, you'll learn what agarose is and how electrophoresis works. You'll also discover the use of agarose in this procedure.
6. Bacterial Transformation: Definition, Steps & Analysis
Bacteria can pick up genes or pieces of DNA from their environment in a process called transformation. In this lesson, we'll explore how this natural process can be exploited in the lab for genetic engineering of bacteria.
7. Polymerase Chain Reaction: Definition & Steps
Have you ever wondered if you could make millions of copies of something in a matter of minutes. We're not discussing a copy machine, but rather a fascinating laboratory procedure called polymerase chain reaction. Learn all about it in this lesson!
8. DNA Cloning: Definition and Process
DNA cloning is used for a variety of purposes, but how does it work? In this video lesson, you will learn about the process of cloning DNA, as well as see examples of how cloning is used in science, medicine, and consumer products.
9. Genomic DNA Analysis
Your genes are what make you who you are, but they also make you a human. Studying genes and genomes allows scientists to better understand not only our own species, but how we are related to other species in the world as well.
10. Practical Applications of DNA Technology
DNA technology is a field that has many applications for our everyday lives. In this video lesson, you will learn about some of the important contributions of DNA technology to industries such as medicine, agriculture and criminal investigation.
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Other chapters within the NYSTCE Biology (006): Practice and Study Guide course
- NYSTCE Biology: Scientific Inquiry
- NYSTCE Biology: Research Methods
- NYSTCE Biology: Measurement & Observation
- NYSTCE Biology: Laboratory Basics
- NYSTCE Biology: Cell Biology
- NYSTCE Biology: Prokaryotic Cells
- NYSTCE Biology: Cell Communication
- NYSTCE Biology: Inorganic Chemistry
- NYSTCE Biology: Organic Compounds
- NYSTCE Biology: Nucleic Acids
- NYSTCE Biology: Enzymes
- NYSTCE Biology: Metabolic Biochemistry
- NYSTCE Biology: Plant Cells
- NYSTCE Biology: DNA Replication
- NYSTCE Biology: Transcription & Translation Process
- NYSTCE Biology: Cell Cycle & Division
- NYSTCE Biology: Classical, Molecular & Population Genetics
- NYSTCE Biology: Genetic Mutations
- NYSTCE Biology: The Origin of Life on Earth
- NYSTCE Biology: Evolution
- NYSTCE Biology: Protists, Fungi & Viruses
- NYSTCE Biology: Plant Biology
- NYSTCE Biology: Plant Reproduction & Growth
- NYSTCE Biology: Animals & Evolution
- NYSTCE Biology: Social Behavior of Animals
- NYSTCE Biology: Circulatory, Respiratory, Digestive, Excretory & Musculoskeletal Systems
- NYSTCE Biology: Nervous, Immune & Endocrine Systems
- NYSTCE Biology: Reproduction & Development
- NYSTCE Biology: Human Reproductive Systems
- NYSTCE Biology: Ecological Principles & Processes
- NYSTCE Biology Flashcards