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Ch 16: Praxis Biology: Biology Lab Techniques

About This Chapter

Explore biology lab techniques with our video lessons and quizzes. You can get ready to demonstrate the competencies of a secondary biology teacher on the Praxis II Biology Content Knowledge exam by working your way through the lessons in this chapter.

Praxis Biology: Biology Lab Techniques - Chapter Summary

Who was Frederick Sanger, and what type of innovative analysis did he develop? You can answer that question, and more, with the lessons in this chapter. Start preparing for your Praxis II test by reviewing the genetic engineering methods scientists use to make human insulin. Next, you can revisit the foundational aspects of plasmids, including multiple cloning sites, selectable markers and origins of replication. Study all the following concepts to prepare for the Praxis II Biology Content Knowledge exam:

  • The use of microscopes in biology research
  • The study of biological processes with the aid of spectrophotometry
  • The use of ligase in recombinant DNA engineering
  • Genetic engineering
  • DNA plasmids
  • Transformation of bacteria
  • Polymerase chain reactions
  • DNA sequencing with the Sanger method

You can experience the lessons through video, written transcript or both. Many lessons include links for additional text lessons covering related topics. Use the lessons as many times as you wish, and you can use the handy video tags to go back to specific portions for extra review.

Praxis Biology: Biology Lab Techniques Objectives

You should expect to answer approximately 38 questions pertinent to the topics outlined above on your Praxis II Biology Content Knowledge exam. About 12 additional questions pertaining to scientific inquiry in general may also apply. Together, those multiple-choice questions compose about 32% of the entire test. Make sure that you understand how DNA research contributes to genetic engineering, reviewing such topics as sequencing and transgenic cells, to be prepared for test day.

Our multiple-choice quizzes offer beneficial practice in the types of questions you might encounter on the Praxis. Your success on the test demonstrates not only your knowledge level, but also your readiness to teach secondary-level biology students.

13 Lessons in Chapter 16: Praxis Biology: Biology Lab Techniques
Test your knowledge with a 30-question chapter practice test
How to Use Spectrophotometry to Understand Beer's Law

1. How to Use Spectrophotometry to Understand Beer's Law

In this lesson we will learn about Beer's Law and how to use spectrophotometry to determine either molar absorbance or concentration in the Beer's Law equation.

How Ligase is Used to Engineer Recombinant DNA

2. How Ligase is Used to Engineer Recombinant DNA

DNA ligase makes recombinant DNA technology possible. In this lesson, you will learn how new versions of genes can be designed for experiments in novel host organisms using DNA ligase.

What is Genetic Engineering? - Definition and Examples

3. What is Genetic Engineering? - Definition and Examples

How do we make the insulin used by diabetic patients? In this lesson, you'll learn the basics of how genetic engineering can be used to transform a bacterial host cell into a genetically-modified organism that produces human insulin.

What is a DNA Plasmid? - Importance to Genetic Engineering

4. What is a DNA Plasmid? - Importance to Genetic Engineering

DNA plasmids play an integral part in most genetic engineering experiments. In this lesson, you'll learn about key features of a plasmid, such as a multiple cloning site, an origin of replication, and a selectable marker.

Restriction Enzymes: Function and Definition

5. Restriction Enzymes: Function and Definition

Restriction enzymes played a critical role in the advent of genetic engineering. In this lesson, you will learn what role restriction enzymes play in creating recombinant DNA.

What is Agarose Gel Electrophoresis?

6. What is Agarose Gel Electrophoresis?

Agarose gel electrophoresis plays a key role in genetic engineering experiments. In this lesson, you'll learn what agarose is and how electrophoresis works. You'll also discover the use of agarose in this procedure.

Ethidium Bromide, Loading Buffer & DNA Ladder: Visualizing DNA and Determining its Size

7. Ethidium Bromide, Loading Buffer & DNA Ladder: Visualizing DNA and Determining its Size

In this lesson, you will learn about the role that ethidium bromide, loading buffers, and DNA ladders play in visualizing DNA and determining the size of DNA fragments in agarose gel electrophoresis.

Agarose Gel Electrophoresis: Equipment & Procedure

8. Agarose Gel Electrophoresis: Equipment & Procedure

This lesson will review the concepts and mechanisms of agarose gel electrophoresis. It will also summarize the equipment needed to perform the procedure for DNA analysis.

Agarose Gel Electrophoresis: Results Analysis

9. Agarose Gel Electrophoresis: Results Analysis

Gel electrophoresis is used to analyze DNA restriction digest and ligation experiments. In this lesson, you will learn how to use a DNA ladder to interpret experimental results.

Bacterial Transformation: Antibiotic Selection and Positive & Negative Controls

10. Bacterial Transformation: Antibiotic Selection and Positive & Negative Controls

The use of antibiotic selection and positive and negative controls are important elements of interpreting data from a bacterial transformation. In this lesson, you will learn how antibiotic selection results in colony formation and how controls help pinpoint the cause of experimental problems.

PCR: Reagents Used in Polymerase Chain Reaction

11. PCR: Reagents Used in Polymerase Chain Reaction

Polymerase chain reaction (PCR) is a biotechnology technique that is used to amplify pieces of DNA. In this lesson, you will learn about five ingredients necessary to perform PCR: template DNA, nucleotides, primers, buffer and Taq polymerase.

PCR: Steps Involved in Polymerase Chain Reaction

12. PCR: Steps Involved in Polymerase Chain Reaction

In this lesson, you will learn about the steps required to amplify DNA during PCR. The lesson will explain the role template DNA, primers, nucleotides, Taq polymerase and PCR buffer play in the denaturation, annealing and extension steps of PCR.

The Sanger Method of DNA Sequencing

13. The Sanger Method of DNA Sequencing

The ability to determine the DNA sequence of an individual is a powerful tool for paternity questions and criminal investigations, among other uses. This lesson will describe one laboratory method that can be used to sequence DNA.

Chapter Practice Exam
Test your knowledge of this chapter with a 30 question practice chapter exam.
Not Taken
Practice Final Exam
Test your knowledge of the entire course with a 50 question practice final exam.
Not Taken

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