Ch 5: Processes and Steps of DNA Replication for the MCAT: Tutoring Solution
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Learning objectives:
- Explore conservative, semi-conservative and dispersive models of DNA replication.
- Examine how the DNA helicase unwinds the double helix in preparation for replication.
- Learn how DNA polymerase and RNA primase initiate DNA replication.
- Find out the significance of leading and lagging strands of DNA.
- Review enzymes and replication bubbles.

1. What Is DNA Replication? - Conservative, Semi-Conservative & Dispersive Models
DNA replication, the process of copying DNA, has three different models to explain its processes, the most well-known of which is the Meselson and Stahl model. Learn about their experiments, as well as how those experiments disproved older models.

2. How Helicase Unwinds the DNA Double Helix in Preparation for Replication
DNA helicase is a vital enzyme that unwinds the DNA double helix and prepares DNA for replication. Learn about this process by exploring the semi-conservative model of DNA replication, explore how DNA helicase unwinds the DNA double helix, and discover the origin of replication and replication fork.

3. How DNA Polymerase and RNA Primase Initiate DNA Replication
DNA replication involves the process of splitting DNA molecules down the middle. Different enzymes, such as DNA polymerase and RNA primase, have different roles in this process, including building daughter strands of DNA and priming for replication. Learn about how DNA replication is started and the enzymes that help it occur.

4. DNA Replication: The Leading Strand and DNA Polymerase Activities
The beginning of DNA replication requires the splitting of two parent DNA strands. DNA polymerase makes short lengths of DNA that are then bound together by DNA ligase. Learn how these enzymes work together to aid in DNA replication.

5. DNA Replication: Review of Enzymes, Replication Bubbles & Leading and Lagging Strands
Enzymes, parent strands, and daughter strands are parts of the process of DNA replication. Learn about the parts of DNA replication, including enzymes, replication bubbles, and leading and lagging strands.

6. Examples of Transcription Regulation in Eukaryotes
Transcription, or gene expression, in eukaryotic cells is regulated by DNA regulatory regions, DNA methylation, and chromatin modifications. Learn about transcription and real-world examples of transcription regulation in eukaryotic cells.
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