Ch 12: Recombinant DNA Technology

About This Chapter

Improve your understanding of recombinant DNA technology and genetic engineering to reinforce what you learned in your biology class or to prepare for an exam. Explore the history of gene manipulation and the technology behind it using these lessons and chapter quizzes.

Recombinant DNA Technology - Chapter Summary

The lessons in this chapter cover the history of recombinant DNA technology. You can explore the origins of this science and the technological breakthroughs that made it possible as well as controversial aspects of genetic engineering. A full lesson is devoted to the steps in the polymerase chain reaction (PCR) and how it affects recombinant DNA. Once you have completed all of these lessons you will be able to:

  • Explain the pros and cons of genetic engineering
  • Describe restriction enzymes
  • Explain how DNA ligase allows experiments to be carried out
  • Fully chart the steps of the PCR
  • Discuss the process of transformation in cells

Each lesson in this chapter includes a video and/or text lesson and an interactive lesson quiz. The videos are designed to succinctly cover the most important points of a topic in an engaging manner while the short quizzes allow you to quickly check yourself after each lesson. Your progress through the chapter will be automatically tracked in your Dashboard, making sure you never lose your place.

5 Lessons in Chapter 12: Recombinant DNA Technology
Test your knowledge with a 30-question chapter practice test
Genetic Manipulation: Definition, Pros & Cons

1. Genetic Manipulation: Definition, Pros & Cons

In this lesson, we'll be looking at genetic manipulation, which is essentially the manipulation of gene sequences in living organisms to alter specific traits. We'll also look at how it works and the controversies surrounding it, and then you'll test your knowledge with a quiz!

Restriction Enzymes: Function and Definition

2. Restriction Enzymes: Function and Definition

Restriction enzymes played a critical role in the advent of genetic engineering. In this lesson, you will learn what role restriction enzymes play in creating recombinant DNA.

How Ligase is Used to Engineer Recombinant DNA

3. How Ligase is Used to Engineer Recombinant DNA

DNA ligase makes recombinant DNA technology possible. In this lesson, you will learn how new versions of genes can be designed for experiments in novel host organisms using DNA ligase.

PCR: Steps Involved in Polymerase Chain Reaction

4. PCR: Steps Involved in Polymerase Chain Reaction

In this lesson, you will learn about the steps required to amplify DNA during PCR. The lesson will explain the role template DNA, primers, nucleotides, Taq polymerase and PCR buffer play in the denaturation, annealing and extension steps of PCR.

Transformed Cells: Definition & Characteristics

5. Transformed Cells: Definition & Characteristics

In this lesson, we'll explore the process of transformation of cells. Keep reading to find out what transformation is, how it was discovered, and how it can potentially be used in gene therapy. After the lesson, take a short quiz to see what you've learned.

Chapter Practice Exam
Test your knowledge of this chapter with a 30 question practice chapter exam.
Not Taken
Practice Final Exam
Test your knowledge of the entire course with a 50 question practice final exam.
Not Taken

Earning College Credit

Did you know… We have over 200 college courses that prepare you to earn credit by exam that is accepted by over 1,500 colleges and universities. You can test out of the first two years of college and save thousands off your degree. Anyone can earn credit-by-exam regardless of age or education level.

To learn more, visit our Earning Credit Page

Transferring credit to the school of your choice

Not sure what college you want to attend yet? has thousands of articles about every imaginable degree, area of study and career path that can help you find the school that's right for you.