About This Chapter
Recombinant DNA Technology - Chapter Summary
The lessons in this chapter cover the history of recombinant DNA technology. You can explore the origins of this science and the technological breakthroughs that made it possible as well as controversial aspects of genetic engineering. A full lesson is devoted to the steps in the polymerase chain reaction (PCR) and how it affects recombinant DNA. Once you have completed all of these lessons you will be able to:
- Explain the pros and cons of genetic engineering
- Describe restriction enzymes
- Explain how DNA ligase allows experiments to be carried out
- Fully chart the steps of the PCR
- Discuss the process of transformation in cells
Each lesson in this chapter includes a video and/or text lesson and an interactive lesson quiz. The videos are designed to succinctly cover the most important points of a topic in an engaging manner while the short quizzes allow you to quickly check yourself after each lesson. Your progress through the chapter will be automatically tracked in your Dashboard, making sure you never lose your place.
1. Genetic Manipulation: Definition, Pros & Cons
In this lesson, we'll be looking at genetic manipulation, which is essentially the manipulation of gene sequences in living organisms to alter specific traits. We'll also look at how it works and the controversies surrounding it, and then you'll test your knowledge with a quiz!
2. Restriction Enzymes: Function and Definition
Restriction enzymes played a critical role in the advent of genetic engineering. In this lesson, you will learn what role restriction enzymes play in creating recombinant DNA.
3. How Ligase is Used to Engineer Recombinant DNA
DNA ligase makes recombinant DNA technology possible. In this lesson, you will learn how new versions of genes can be designed for experiments in novel host organisms using DNA ligase.
4. PCR: Steps Involved in Polymerase Chain Reaction
In this lesson, you will learn about the steps required to amplify DNA during PCR. The lesson will explain the role template DNA, primers, nucleotides, Taq polymerase and PCR buffer play in the denaturation, annealing and extension steps of PCR.
5. Transformed Cells: Definition & Characteristics
In this lesson, we'll explore the process of transformation of cells. Keep reading to find out what transformation is, how it was discovered, and how it can potentially be used in gene therapy. After the lesson, take a short quiz to see what you've learned.
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Other chapters within the HSC Biology: Exam Prep & Syllabus course
- Scientific Investigations Overview
- Scientific Validity
- Data Collection & Analysis in Science
- Scientific Presentations
- Internal & External Environments
- Gas & Nutrient Transport in Animals & Plants
- Regulation in Animals & Plants
- Chromosomal Structure & Inheritance
- Mendel & Gene Expression
- Mechanisms of Genetic Change
- DNA Replication & Protein Synthesis
- Biotechnology Basics
- Genetic Engineering & Reproductive Technologies
- Classification Hierarchy
- Health & Disease
- Immune Response
- Nervous System & Sensory Mechanisms
- Anatomy & Disorders of the Eye
- Sound & Hearing
- Evolutionary Change
- Time & Human Evolution
- History of Botany
- Photosynthesis Basics
- HSC Biology Flashcards