About This Chapter
Serological & Diagnostic Reactions - Chapter Summary
This online science chapter outlines several diagnostic and serological reactions in microbiology. Work through the chapter at your own pace to review concepts like immunological and radioimmunoassay methods, viral replication, agglutination and complement fixation. To help you retain these concepts, we've included interactive self-assessment quizzes throughout the chapter. You can study these types of reactions at any time using your computer or mobile device. Feel free to reach out to our instructors if you have any questions about the material. Reviewing the chapter can help you:
- Identify immunological methods that are commonly used in biology labs
- Define the radioimmunoassay method
- Assess the application of enzyme-linked immunosorbent assay
- Evaluate the process of viral replication and antibody neutralization
- Recognize examples of agglutination in microbiology
- Analyze examples of precipitation reactions and complement fixation
- Differentiate between monoclonal and polyclonal antibodies
- Name reagents that are used in polymerase chain reactions
- Understand the use of gene probes
1. Immunological Methods Used in Biology Labs
In this lesson, we will discuss immunological methods used in cellular and molecular biology labs. These methods are based on antigen-antibody interactions and include western blots, immunofluorescence, and immunohistochemistry.
2. Radioimmunoassay: Definition & Method
How can scientists measure substances in a person's blood? In this lesson you'll learn about radioimmunoassays, a commonly used method of detecting substances in blood, especially antigens.
3. Enzyme-Linked Immunosorbent Assay: Definition & Application
The enzyme-linked immunosorbent assay is a tool used by scientists to study protein interactions. Read on to learn about the different types of assays and how they work.
4. Antibody Neutralization & Viral Replication
How can a single antibody stop a virus from copying itself repeatedly and taking over? Read on to learn how antibodies neutralize viruses and stop them from replicating.
5. Agglutination in Microbiology: Definition & Examples
Discover the importance of agglutination, a process used by the immune system to inactivate invading foreign substances. Learn how agglutination is a tool used in the health care industry to diagnose illness.
6. Monoclonal vs. Polyclonal Antibodies: Definition & Production
In this lesson, you'll discover what monoclonal and polyclonal antibodies are. In addition, you'll learn how they're made, their uses, and some of the differences between them.
7. PCR: Reagents Used in Polymerase Chain Reaction
Polymerase chain reaction (PCR) is a biotechnology technique that is used to amplify pieces of DNA. In this lesson, you will learn about five ingredients necessary to perform PCR: template DNA, nucleotides, primers, buffer and Taq polymerase.
8. Gene Probe: Definition, Use & Example
In this lesson we'll learn the basics about gene probes. We'll discuss what these molecular biology tools are and how they work to identify microorganisms in microbiology.
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Other chapters within the UExcel Microbiology: Study Guide & Test Prep course
- Early History of Microbiology
- Fundamentals of Microbiology
- Microbiology Laboratory Procedures
- Understanding Genetics & DNA
- Understanding Metabolism
- Bacterial Biology
- Understanding Diseases
- Protozoan Diseases
- Understanding Viruses
- Overview of DNA Viruses
- Overview of RNA Viruses
- Overview of Fungal Infections
- Illnesses & Infections of the GI Tract
- Overview of Bacterial STDs
- Understanding Blood-Borne Bacterial Diseases
- Respiratory Bacterial Infections
- Bacterial Skin and Wound Infections
- Immunology Basics
- Structure & Function of the Immune System
- Overview of Antigens
- Disorders of the Immune System
- Antimicrobial Drugs Overview
- Sterilization & Antiseptic Methods
- Chemical Methods of Microbial Control
- Effectiveness of Antiseptics & Disinfectants
- Microbiology & the Environment
- Food & Industrial Microbiology Overview
- UExcel Microbiology Flashcards